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The putative transcriptional activator MSN1 promotes chromium accumulation in Saccharomyces cerevisiae

Title
The putative transcriptional activator MSN1 promotes chromium accumulation in Saccharomyces cerevisiae
Authors
Chang K.S.Won J.I.Lee M.R.Lee C.E.Kim K.H.Park K.Y.Kim S.-K.Lee J.S.Hwang S.
Ewha Authors
이준승
SCOPUS Author ID
이준승scopusscopus
Issue Date
2003
Journal Title
Molecules and Cells
ISSN
1016-8478JCR Link
Citation
vol. 16, no. 3, pp. 291 - 296
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
Yeast is a good system for studying molecular mechanisms of metal tolerance. Using a mini-Tn mutagenized yeast pool, we isolated a chromate-tolerant mutant, CrT9, that displayed metal-specific tolerance since it was only tolerant to Cr(VI), not to Cr(III), Cd, As, or Fe. The Cr-tolerance of CrT9 appeared to be due to reduced Cr accumulation as it accumulated only 56% as much as WT (Y800). Using IPCR (inverse PCR), we found that the mini-Tn had been inserted at nt 741 of the transcriptional activator, MSN1. MSN1 is a multifunctional protein involved in invertase activity, iron uptake, starch degradation, pseudohyphal growth, and osmotic gene expression. We found that there was only one mini-Tn insertion in CrT9 since MSN1 and mini-Tn probes hybridized to the same DNA fragment, and the MSN1 probe detected an enlarged MSN1 mRNA. When we over-expressed MSN1 in CrT9 and WT, both accumulated larger amounts of Cr. We conclude that Cr accumulation in S. cerevisiae is promoted by the transcriptional activator MSN1. ©KSMCB 2003.
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자연과학대학 > 생명과학전공 > Journal papers
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