Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이준승 | * |
dc.date.accessioned | 2016-08-28T11:08:34Z | - |
dc.date.available | 2016-08-28T11:08:34Z | - |
dc.date.issued | 2003 | * |
dc.identifier.issn | 1016-8478 | * |
dc.identifier.other | OAK-1784 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219366 | - |
dc.description.abstract | Yeast is a good system for studying molecular mechanisms of metal tolerance. Using a mini-Tn mutagenized yeast pool, we isolated a chromate-tolerant mutant, CrT9, that displayed metal-specific tolerance since it was only tolerant to Cr(VI), not to Cr(III), Cd, As, or Fe. The Cr-tolerance of CrT9 appeared to be due to reduced Cr accumulation as it accumulated only 56% as much as WT (Y800). Using IPCR (inverse PCR), we found that the mini-Tn had been inserted at nt 741 of the transcriptional activator, MSN1. MSN1 is a multifunctional protein involved in invertase activity, iron uptake, starch degradation, pseudohyphal growth, and osmotic gene expression. We found that there was only one mini-Tn insertion in CrT9 since MSN1 and mini-Tn probes hybridized to the same DNA fragment, and the MSN1 probe detected an enlarged MSN1 mRNA. When we over-expressed MSN1 in CrT9 and WT, both accumulated larger amounts of Cr. We conclude that Cr accumulation in S. cerevisiae is promoted by the transcriptional activator MSN1. ©KSMCB 2003. | * |
dc.language | English | * |
dc.title | The putative transcriptional activator MSN1 promotes chromium accumulation in Saccharomyces cerevisiae | * |
dc.type | Article | * |
dc.relation.issue | 3 | * |
dc.relation.volume | 16 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 291 | * |
dc.relation.lastpage | 296 | * |
dc.relation.journaltitle | Molecules and Cells | * |
dc.identifier.wosid | WOS:000187603900004 | * |
dc.identifier.scopusid | 2-s2.0-1442356428 | * |
dc.author.google | Chang K.S. | * |
dc.author.google | Won J.I. | * |
dc.author.google | Lee M.R. | * |
dc.author.google | Lee C.E. | * |
dc.author.google | Kim K.H. | * |
dc.author.google | Park K.Y. | * |
dc.author.google | Kim S.-K. | * |
dc.author.google | Lee J.S. | * |
dc.author.google | Hwang S. | * |
dc.contributor.scopusid | 이준승(7601452195) | * |
dc.date.modifydate | 20240423081003 | * |