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Triacylglycerol, 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol isolated from bovine udder and its synthetic enantiomer can potentiate the mitogenic activity for mouse peritoneal macrophages
- Title
- Triacylglycerol, 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol isolated from bovine udder and its synthetic enantiomer can potentiate the mitogenic activity for mouse peritoneal macrophages
- Authors
- Suh J.S.; Kwon J.; Eun J.S.; Lee Y.J.; Limb J.K.; Ko S.Y.; Han S.Y.; Bae Y.S.; Jhon G.J.
- Ewha Authors
- 전길자; 배윤수; 고수영; 한소엽; 임진경
- SCOPUS Author ID
- 전길자; 배윤수; 고수영; 한소엽; 임진경
- Issue Date
- 2003
- Journal Title
- Cellular Physiology and Biochemistry
- ISSN
- 1015-8987
- Citation
- Cellular Physiology and Biochemistry vol. 13, no. 6, pp. 415 - 422
- Indexed
- SCI; SCIE; SCOPUS
- Document Type
- Article
- Abstract
- A factor stimulating a mitogenic activity of peritoneal macrophages is purified from bovine udder. It is identified as a triglyceride, 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (rac-MADG). In this study, its enantiomers, R-(+)-and S-(-)-1-palmitoyl-2-linoleoyl-3-acetylglycerol (R-(+)-MADG, S-(-)-MADG) are synthesized. Among them, R-(+)-MADG enantiomer turns out to increase a mitogenic activity in mouse peritoneal macrophages. Also, (S)-(-)-MADG shows a low mitogenic activity. Treatment of a macrophage with R-(+)-MADG increases reactive oxygen species (ROS). Furthermore, treatment of macrophages with antioxidant, N-acetyl-L-cysteine (NAC), suppresses the R-(+)-MADG-dependent macrophage proliferation. Results show that the generation of ROS induces in R-(+)-MADG-dependent cell signaling. Treatment of a macrophage with R-(+)-MADG increases the activity of protein kinase C (PKC). Treatment of macrophages with calphostin C inhibits R-(+)-MADG-induced macrophage proliferation. Results suggest that R-(+)-MADG enhances the activity of protein kinase C (PKC) and stimulates the macrophage growth. In conclusions, R-(+)-MADG accelerates the production of ROS and increases the activity of PKC to eventually stimulate macrophage cell growth. The existence of rac-MADG in bovine udder and milk provides passive protection for the neonate and immunostimulatory capabilities. Copyright © 2003 S. Karger AG, Basel.
- DOI
- 10.1159/000075129
- Appears in Collections:
- 자연과학대학 > 화학·나노과학전공 > Journal papers
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