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FLASH Coordinates NF-κB Activity via TRAF2
- Title
- FLASH Coordinates NF-κB Activity via TRAF2
- Authors
- Choi Y.-H.; Kim K.-B.; Kim H.-H.; Hong G.-S.; Kwon Y.-K.; Chung C.-W.; Park Y.-M.; Shen Z.-J.; Kim B.J.; Lee S.-Y.; Jung Y.-K.
- Ewha Authors
- 이수영
- SCOPUS Author ID
- 이수영
- Issue Date
- 2001
- Journal Title
- Journal of Biological Chemistry
- ISSN
- 0021-9258
- Citation
- Journal of Biological Chemistry vol. 276, no. 27, pp. 25073 - 25077
- Indexed
- SCI; SCIE; SCOPUS
- Document Type
- Article
- Abstract
- FLASH is a protein recently shown to interact with the death effector domain of caspase-8 and is likely to be a component of the death-inducing signaling complex in receptor-mediated apoptosis. Here we show that anti-sense oligonucleotide-induced inhibition of FLASH expression abolished TNF-α-induced activation of NF-κB in HEK293 cells, as determined by luciferase reporter gene expression driven by a NF-κB responsive promoter. Conversely, overexpression of FLASH dose-dependently activated NF-κB, an effect suppressed by dominant negative mutants of TRAF2, NIK, and IKKα, and partially by those of TRAF5 and TRAF6. TRAF2 was co-immunoprecipitated with FLASH from the cell extracts of HEK293 cells or HeLa cells stably expressing exogenous FLASH (HeLa/HA-FLASH). Furthermore, serial deletion mapping demonstrated that a domain spanning the residues 856-1191 of FLASH activated NF-κB as efficiently as the full-length and could directly bind to TRAF2 in vitro and in the transfected cells. Taken together, these results suggest that FLASH coordinates downstream NF-κB activity via a TRAF2-dependent pathway in the TNF-α signaling.
- DOI
- 10.1074/jbc.M102941200
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
- Files in This Item:
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