Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이수영 | * |
dc.date.accessioned | 2016-08-28T11:08:44Z | - |
dc.date.available | 2016-08-28T11:08:44Z | - |
dc.date.issued | 2001 | * |
dc.identifier.issn | 0021-9258 | * |
dc.identifier.other | OAK-772 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/218831 | - |
dc.description.abstract | FLASH is a protein recently shown to interact with the death effector domain of caspase-8 and is likely to be a component of the death-inducing signaling complex in receptor-mediated apoptosis. Here we show that anti-sense oligonucleotide-induced inhibition of FLASH expression abolished TNF-α-induced activation of NF-κB in HEK293 cells, as determined by luciferase reporter gene expression driven by a NF-κB responsive promoter. Conversely, overexpression of FLASH dose-dependently activated NF-κB, an effect suppressed by dominant negative mutants of TRAF2, NIK, and IKKα, and partially by those of TRAF5 and TRAF6. TRAF2 was co-immunoprecipitated with FLASH from the cell extracts of HEK293 cells or HeLa cells stably expressing exogenous FLASH (HeLa/HA-FLASH). Furthermore, serial deletion mapping demonstrated that a domain spanning the residues 856-1191 of FLASH activated NF-κB as efficiently as the full-length and could directly bind to TRAF2 in vitro and in the transfected cells. Taken together, these results suggest that FLASH coordinates downstream NF-κB activity via a TRAF2-dependent pathway in the TNF-α signaling. | * |
dc.language | English | * |
dc.title | FLASH Coordinates NF-κB Activity via TRAF2 | * |
dc.type | Article | * |
dc.relation.issue | 27 | * |
dc.relation.volume | 276 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 25073 | * |
dc.relation.lastpage | 25077 | * |
dc.relation.journaltitle | Journal of Biological Chemistry | * |
dc.identifier.doi | 10.1074/jbc.M102941200 | * |
dc.identifier.wosid | WOS:000169800700086 | * |
dc.identifier.scopusid | 2-s2.0-0035816722 | * |
dc.author.google | Choi Y.-H. | * |
dc.author.google | Kim K.-B. | * |
dc.author.google | Kim H.-H. | * |
dc.author.google | Hong G.-S. | * |
dc.author.google | Kwon Y.-K. | * |
dc.author.google | Chung C.-W. | * |
dc.author.google | Park Y.-M. | * |
dc.author.google | Shen Z.-J. | * |
dc.author.google | Kim B.J. | * |
dc.author.google | Lee S.-Y. | * |
dc.author.google | Jung Y.-K. | * |
dc.contributor.scopusid | 이수영(53980218900;7409697278) | * |
dc.date.modifydate | 20240415140424 | * |