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Lamivudine production via enantioselective deamination by thermostable Bacillus caldolyticus cytidine deaminase

Title
Lamivudine production via enantioselective deamination by thermostable Bacillus caldolyticus cytidine deaminase
Authors
Woo J.-H.Shin H.-J.Kim T.-H.Ghim S.-Y.Jeong L.-S.Kim J.-G.Song B.-H.
Ewha Authors
정낙신
Issue Date
2001
Journal Title
Biotechnology Letters
ISSN
0141-5492JCR Link
Citation
vol. 23, no. 2, pp. 131 - 135
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
To decrease the costs of producing the anti-HIV drug, lamivudine, an enzymatic conversion process was developed instead of the traditional chemical method. Thermostable cytidine deaminase was over-produced by cloning the cdd gene into E. coli JF611/pCJH53 from Bacillus caldolyticus. The purified cytidine deaminase was recovered from the lysate of the recombinant E. coli JF611/pCJH53 by removing heat-denatured proteins and eluting sequential chromatography. When the enzyme was used to deaminate (-)-β-L-(2R,5S)- and (+)-β-D-(2S,5R)-1,3-oxathiolanyl-cytosine, about 68% of the (+)-β-D-(2S,5R)-1,3-oxathiolanyl-cytosine was deaminated into the corresponding (+)-thiauridine maximally.
DOI
10.1023/A:1010353502346
Appears in Collections:
약학대학 > 약학과 > Journal papers
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