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Lamivudine production via enantioselective deamination by thermostable Bacillus caldolyticus cytidine deaminase
- Lamivudine production via enantioselective deamination by thermostable Bacillus caldolyticus cytidine deaminase
- Woo J.-H.; Shin H.-J.; Kim T.-H.; Ghim S.-Y.; Jeong L.-S.; Kim J.-G.; Song B.-H.
- Ewha Authors
- Issue Date
- Journal Title
- Biotechnology Letters
- vol. 23, no. 2, pp. 131 - 135
- SCI; SCIE; SCOPUS
- To decrease the costs of producing the anti-HIV drug, lamivudine, an enzymatic conversion process was developed instead of the traditional chemical method. Thermostable cytidine deaminase was over-produced by cloning the cdd gene into E. coli JF611/pCJH53 from Bacillus caldolyticus. The purified cytidine deaminase was recovered from the lysate of the recombinant E. coli JF611/pCJH53 by removing heat-denatured proteins and eluting sequential chromatography. When the enzyme was used to deaminate (-)-β-L-(2R,5S)- and (+)-β-D-(2S,5R)-1,3-oxathiolanyl-cytosine, about 68% of the (+)-β-D-(2S,5R)-1,3-oxathiolanyl-cytosine was deaminated into the corresponding (+)-thiauridine maximally.
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