Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 정낙신 | - |
dc.date.accessioned | 2016-08-28T11:08:34Z | - |
dc.date.available | 2016-08-28T11:08:34Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.other | OAK-598 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/218735 | - |
dc.description.abstract | To decrease the costs of producing the anti-HIV drug, lamivudine, an enzymatic conversion process was developed instead of the traditional chemical method. Thermostable cytidine deaminase was over-produced by cloning the cdd gene into E. coli JF611/pCJH53 from Bacillus caldolyticus. The purified cytidine deaminase was recovered from the lysate of the recombinant E. coli JF611/pCJH53 by removing heat-denatured proteins and eluting sequential chromatography. When the enzyme was used to deaminate (-)-β-L-(2R,5S)- and (+)-β-D-(2S,5R)-1,3-oxathiolanyl-cytosine, about 68% of the (+)-β-D-(2S,5R)-1,3-oxathiolanyl-cytosine was deaminated into the corresponding (+)-thiauridine maximally. | - |
dc.language | English | - |
dc.title | Lamivudine production via enantioselective deamination by thermostable Bacillus caldolyticus cytidine deaminase | - |
dc.type | Article | - |
dc.relation.issue | 2 | - |
dc.relation.volume | 23 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 131 | - |
dc.relation.lastpage | 135 | - |
dc.relation.journaltitle | Biotechnology Letters | - |
dc.identifier.doi | 10.1023/A:1010353502346 | - |
dc.identifier.wosid | WOS:000166224900008 | - |
dc.identifier.scopusid | 2-s2.0-0035133244 | - |
dc.author.google | Woo J.-H. | - |
dc.author.google | Shin H.-J. | - |
dc.author.google | Kim T.-H. | - |
dc.author.google | Ghim S.-Y. | - |
dc.author.google | Jeong L.-S. | - |
dc.author.google | Kim J.-G. | - |
dc.author.google | Song B.-H. | - |
dc.contributor.scopusid | 정낙신(16028528200) | - |
dc.date.modifydate | 20211210153610 | - |