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Mass production of poly(3-hydroxybutyrate) by fed-batch cultures of Ralstonia eutropha with nitrogen and phosphate limitation

Title
Mass production of poly(3-hydroxybutyrate) by fed-batch cultures of Ralstonia eutropha with nitrogen and phosphate limitation
Authors
Ryu H.W.Cho K.-S.Kim B.S.Chang Y.K.Chang H.N.Shim H.J.
Ewha Authors
조경숙
SCOPUS Author ID
조경숙scopus
Issue Date
1999
Journal Title
Journal of Microbiology and Biotechnology
ISSN
1017-7825JCR Link
Citation
vol. 9, no. 6, pp. 751 - 756
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
For mass production of poly(3-hydroxybutyrate) (PHB), high cell density cultures of Ralstonia eutropha were carded out in 2.5-1 and 60-1 fermentors by two fed-batch culture techniques of nitrogen and phosphate limitation. When the nitrogen limitation technique was employed using both an on-line glucose monitoring and control system, a high concentration level of PHB (121 g/l) was obtained in the small-scale fermentor of 2.5-1. However, the PHB concentration obtained in a large-scale fermentor of 60-1 only turned out to be 60 g/l. In contrast, when another fed-batch culture technique of the phosphate-limitation employing dissolved oxygen (DO) stat glucose feeding was used, a large amount of PHB was successfully produced in both 60-1 and 2.5-1 fermentors. In a 2.5-1 fermentor, concentrations of PHB and cells obtained in 58 h were 175 and 210 g/l, respectively, which corresponded to the PHB productivity level of 3.02 g/l/h. In a 60-1 fermentor, a final cell concentration of 221 g/l and a PHB concentration of 180 g/l with PHB productivity level of 3.75 g/l/h were obtained in 48 h. PHB content and yield from glucose were 81% and 0.38 g PHB/g glucose, respectively. These data suggest that the phosphate limitation technique is more effective compared to nitrogen limitation in the mass production of PHB by R. eutropha of a large scale.
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엘텍공과대학 > 환경공학전공 > Journal papers
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