Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 조경숙 | * |
dc.date.accessioned | 2016-08-28T11:08:22Z | - |
dc.date.available | 2016-08-28T11:08:22Z | - |
dc.date.issued | 1999 | * |
dc.identifier.issn | 1017-7825 | * |
dc.identifier.other | OAK-350 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/218595 | - |
dc.description.abstract | For mass production of poly(3-hydroxybutyrate) (PHB), high cell density cultures of Ralstonia eutropha were carded out in 2.5-1 and 60-1 fermentors by two fed-batch culture techniques of nitrogen and phosphate limitation. When the nitrogen limitation technique was employed using both an on-line glucose monitoring and control system, a high concentration level of PHB (121 g/l) was obtained in the small-scale fermentor of 2.5-1. However, the PHB concentration obtained in a large-scale fermentor of 60-1 only turned out to be 60 g/l. In contrast, when another fed-batch culture technique of the phosphate-limitation employing dissolved oxygen (DO) stat glucose feeding was used, a large amount of PHB was successfully produced in both 60-1 and 2.5-1 fermentors. In a 2.5-1 fermentor, concentrations of PHB and cells obtained in 58 h were 175 and 210 g/l, respectively, which corresponded to the PHB productivity level of 3.02 g/l/h. In a 60-1 fermentor, a final cell concentration of 221 g/l and a PHB concentration of 180 g/l with PHB productivity level of 3.75 g/l/h were obtained in 48 h. PHB content and yield from glucose were 81% and 0.38 g PHB/g glucose, respectively. These data suggest that the phosphate limitation technique is more effective compared to nitrogen limitation in the mass production of PHB by R. eutropha of a large scale. | * |
dc.language | English | * |
dc.title | Mass production of poly(3-hydroxybutyrate) by fed-batch cultures of Ralstonia eutropha with nitrogen and phosphate limitation | * |
dc.type | Article | * |
dc.relation.issue | 6 | * |
dc.relation.volume | 9 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 751 | * |
dc.relation.lastpage | 756 | * |
dc.relation.journaltitle | Journal of Microbiology and Biotechnology | * |
dc.identifier.wosid | WOS:000084577500010 | * |
dc.identifier.scopusid | 2-s2.0-6744266675 | * |
dc.author.google | Ryu H.W. | * |
dc.author.google | Cho K.-S. | * |
dc.author.google | Kim B.S. | * |
dc.author.google | Chang Y.K. | * |
dc.author.google | Chang H.N. | * |
dc.author.google | Shim H.J. | * |
dc.contributor.scopusid | 조경숙(7403957095) | * |
dc.date.modifydate | 20240322131338 | * |