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Nucleotide and deduced amino acid sequences of rat myosin binding protein H (MyBP-H)

Title
Nucleotide and deduced amino acid sequences of rat myosin binding protein H (MyBP-H)
Authors
Jung J.Oh J.Lee K.
Ewha Authors
이경림
SCOPUS Author ID
이경림scopus
Issue Date
1998
Journal Title
Archives of Pharmacal Research
ISSN
0253-6269JCR Link
Citation
vol. 21, no. 6, pp. 712 - 717
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
The complete nucleotide sequence of the cDNA clone encoding rat skeletal muscle myosin-binding protein H (MyBP-H) was determined and amino acid sequence was deduced from the nucleotide sequence (GenBank accession number AF077338). The full-length cDNA of 1782 base pairs(bp) contains a single open reading frame of 1454 bp encoding a rat MyBP-H protein of the predicted molecular mass 52.7kDa and includes the common consensus 'CA_ _TG' protein binding motif. The cDNA sequence of rat MyBP-H show 92%, 84% and 41% homology with those of mouse, human and chicken, respectively. The protein contains tandem internal motifs array (-FN III-Ig C2-FN III- Ig C2-) in the C-terminal region which resembles to the immunoglobulin superfamily C2 and fibronectin type III motifs. The amino acid sequence of the C-terminal Ig C2 was highly conserved among MyBPs family and other thick filament binding proteins, suggesting that the C-terminal Ig C2 might play an important role in its function. All proteins belonging to MyBP-H member contains 'RKPS' sequence which is assumed to be cAMP- and cGMP-dependent protein kinase A phosphorylation site. Computer analysis of the primary sequence of rat MyBP-H predicted 11 protein kinase C (PKC) phosphorylation site, 7 casein kinase II (CK2) phosphorylation site and 4 N-myristoylation site.
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약학대학 > 약학과 > Journal papers
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