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A Selective Imidazoline-2-thione-Bearing Two-Photon Fluorescent Probe for Hypochlorous Acid in Mitochondria

Title
A Selective Imidazoline-2-thione-Bearing Two-Photon Fluorescent Probe for Hypochlorous Acid in Mitochondria
Authors
Xu, QinglingHeo, Cheol HoKim, Jin A.Lee, Hye SueHu, YingKim, DayoungSwamy, Kunemadihalli Mathada KotraiahKim, GyoungmiNam, Sang-JipKim, Hwan MyungYoon, Juyoung
Ewha Authors
윤주영남상집
SCOPUS Author ID
윤주영scopus; 남상집scopus
Issue Date
2016
Journal Title
ANALYTICAL CHEMISTRY
ISSN
0003-2700JCR Link1520-6882JCR Link
Citation
vol. 88, no. 12, pp. 6615 - 6620
Publisher
AMER CHEMICAL SOC
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Hypochlorite (OCl-) plays a key role in the immune system and is involved in various diseases. Accordingly, direct detection of endogenous OCl- at the subcellular level is important for understanding inflammation and cellular apoptosis. In the current study, a two-photon fluorescent off/on probe (PNIS) bearing imidazoline-2-thione as an OCl- recognition unit and triphenylphosphine (TPP) as a mitochondrial-targeting group was synthesized and examined for its ability to image mitochondrial OCl- in situ. This probe, based on the specific reaction between imidazoline-2-thione and OCl-, displayed a selective fluorescent off/on response to OCl- with the various reactive oxygen species in a physiological medium. PNIS was successfully applied to image of endogenously produced mitochondrial OCl- in live RAW 264.7 cells-via two-photon microscopy.
DOI
10.1021/acs.analchem.6b01738
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
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