View : 60 Download: 0

Cytotoxic effects of one-step self-etching adhesives on an odontoblast cell line

Title
Cytotoxic effects of one-step self-etching adhesives on an odontoblast cell line
Authors
Lee, YoonAn, So-YounPark, Yoon-JungYu, Frank H.Park, Joo-CheolSeo, Deog-Gyu
Ewha Authors
박윤정
SCOPUS Author ID
박윤정scopus
Issue Date
2016
Journal Title
SCANNING
ISSN
0161-0457JCR Link1932-8745JCR Link
Citation
vol. 38, no. 1, pp. 36 - 42
Keywords
cytotoxicityflow cytometric apoptosis assayMTTodontoblastone-step self-etching adhesivescanning electron microscopy
Publisher
WILEY-BLACKWELL
Indexed
SCI; SCIE WOS
Abstract
The aim of this study was to evaluate the cytotoxic effects of one-step self-etching adhesives. Cells from an immortalized mouse odontoblast cell line (MDPC-23) were cultured with six different dental adhesive systems (diluted to concentrations of 0.5% for 4h): Adper Easy Bond (EB), Xeno V (XV), iBond (IB), AdheSE One (AO), Clearfil SE primer (CS), and Adper Single Bond 2 (SB). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and flow cytometric apoptosis assays were used to evaluate cell viability and the rate of apoptosis. The odontoblasts were also examined under a scanning electron microscope. While all of the cultures with adhesives showed reduced viability, the viabilities in the IB and SB groups were not significantly different from the control group. Although increased apoptosis rates were observed in all of the cultures with adhesives, the rate in the SB group was not significantly different from the rate in the control. The control group showed the lowest apoptosis rate followed by the SB, AO, IB, EB, XV, and CS groups. When examined under a scanning electron microscope, control odontoblasts and the SB group exhibited relatively large cytoplasmic extensions. In contrast, in the EB and CS groups, fewer fibroblasts remained adhered to the plate surface. Cytoplasmic membrane shrinkage and cell-free areas with residual membrane fragments from dead cells were observed. In conclusion, all cultures with one-step self-etching adhesives showed increased apoptotic activity. SB, an etch-and-rinse adhesive, was comparable to the control group, and CS and EB showed the lowest odontoblast viabilities according to the MTT assay. SCANNING 38:36-42, 2016. (c) 2015 Wiley Periodicals, Inc.
DOI
10.1002/sca.21236
Appears in Collections:
신산업융합대학 > 식품영양학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE