Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 장유진 | - |
dc.date.accessioned | 2016-08-27T04:08:39Z | - |
dc.date.available | 2016-08-27T04:08:39Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0024-3205 | - |
dc.identifier.issn | 1879-0631 | - |
dc.identifier.other | OAK-15108 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/217308 | - |
dc.description.abstract | Aims: Previous reports have demonstrated that the adipocyte-derived peptide adiponectin is closely associated with insulin resistance due to its insulin-sensitizing and anti-inflammatory properties in peripheral tissues; however the autocrine effects of adiponectin remain elusive. This study investigated regulatory effects of adiponectin on glucose transport and insulin signaling in insulin-sensitive or insulin-resistant 3T3-L1 adipocytes. Main methods: 3T3-L1 fibroblasts were transfected with non-target or adiponectin (ADN) siRNA and differentiated. Chronic treatment with insulin (24 h, 100 nM) was employed to induce insulin resistance in differentiated adipocytes. Insulin-stimulated glucose transport was measured and protein and mRNA levels were assessed by Western blot and RT-PCR. Key findings: Prolonged incubation with insulin significantly reduced insulin-stimulated glucose uptake, suggesting the development of insulin resistance and adiponectin mRNA expression. In this insulin-resistant condition, adiponectin deletion did not alter insulin-stimulated glucose uptake. In insulin-sensitive adipocytes, adiponectin ablation reduced insulin-stimulated glucose uptake, expression of IRS-1 and GLUT4, and GLUT4 translocation to the membrane. Adiponectin knockdown did not affect the activation of Ala and p38MAPK (phosphorylation form/total form), but significantly decreased the activation of AMPK in insulin-responsive adipocytes. Significance: Adiponectin deficiency suppresses insulin-induced glucose uptake, insulin signaling, and the AMPK pathway only in insulin-responsive 3T3-L1 adipocytes. (C) 2015 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.subject | Adipocytes | - |
dc.subject | Adiponectin | - |
dc.subject | Insulin signaling | - |
dc.subject | Insulin resistance | - |
dc.title | Adiponectin deletion impairs insulin signaling in insulin-sensitive but not insulin-resistant 3T3-L1 adipocytes | - |
dc.type | Article | - |
dc.relation.volume | 132 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 93 | - |
dc.relation.lastpage | 100 | - |
dc.relation.journaltitle | LIFE SCIENCES | - |
dc.identifier.doi | 10.1016/j.lfs.2015.02.013 | - |
dc.identifier.wosid | WOS:000355889900014 | - |
dc.identifier.scopusid | 2-s2.0-84930621356 | - |
dc.author.google | Chang, Eugene | - |
dc.author.google | Choi, Jung Mook | - |
dc.author.google | Park, Se Eun | - |
dc.author.google | Rhee, Eun-Jung | - |
dc.author.google | Lee, Won-Young | - |
dc.author.google | Oh, Ki-Won | - |
dc.author.google | Park, Sung-Woo | - |
dc.author.google | Park, Cheol-Young | - |
dc.contributor.scopusid | 장유진(30367468300) | - |
dc.date.modifydate | 20230411105300 | - |