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Cytosolic Hsp60 orchestrates the survival and inflammatory responses of vascular smooth muscle cells in injured aortic vessels
- Title
- Cytosolic Hsp60 orchestrates the survival and inflammatory responses of vascular smooth muscle cells in injured aortic vessels
- Authors
- Choi, Boae; Choi, Mina; Park, Charny; Lee, Eun Kyung; Kang, Dong Hoon; Lee, Doo Jae; Yeom, Jae Yoon; Jung, Yeonjoo; Kim, Jaesang; Lee, Sanghyuk; Kang, Sang Won
- Ewha Authors
- 이상혁; 강상원; 김재상; 이두재; 정연주; 강동훈
- SCOPUS Author ID
- 이상혁; 강상원; 김재상; 이두재; 정연주; 강동훈
- Issue Date
- 2015
- Journal Title
- CARDIOVASCULAR RESEARCH
- ISSN
- 0008-6363
1755-3245
- Citation
- CARDIOVASCULAR RESEARCH vol. 106, no. 3, pp. 498 - 508
- Keywords
- Hsp60; Vascular smooth muscle cells; NF-kappa B; Inflammation; Restenosis
- Publisher
- OXFORD UNIV PRESS
- Indexed
- SCI; SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Aims Pro-inflammatory response of vascular smooth muscle cells (VSMCs) is triggered by endothelial damage and a causative step for thrombosis and neointimal thickening in the injured arterial vessels. Therefore, we investigate a role of cytosolic Hsp60 as a novel pro-inflammatory mediator in VSMCs. Methods and results Hsp60 was detected in the cytosol of VSMCs. The selective depletion of cytosolic Hsp60 in VSMCs reduced the I kappa B kinase activation, repressed the induction of nuclear factor (NF)-kappa B-dependent survival genes (MnSOD and Bfl-1/A1), and enhanced apoptotic death in response to TNF-alpha Moreover, a quantitative RNA sequencing revealed that the expression of 75 genes among the 774 TNF-alpha-inducible genes was significantly reduced by the depletion of cytosolic Hsp60. In particular, the expression of pro-inflammatory cytokines/chemokines, such as CCL2, CCL20, and IL-6, was regulated by the cytosolic Hsp60 in VSMCs. Finally, the depletion of cytosolic Hsp60 markedly inhibited the neointimal thickening in the balloon-injured arterial vessels by inducing apoptotic cell death and inhibiting chemokine production. Conclusions This study provides the first evidence that cytosolic Hsp60 could be a therapeutic target for preventing VSMC hyperplasia and inflammatory response in the injured vessels.
- DOI
- 10.1093/cvr/cvv130
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
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