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Sasa quelpaertensis Leaf Extract Inhibits Colon Cancer by Regulating Cancer Cell Stemness in Vitro and in Vivo

Title
Sasa quelpaertensis Leaf Extract Inhibits Colon Cancer by Regulating Cancer Cell Stemness in Vitro and in Vivo
Authors
Min, Soo JinLim, Ji YeKim, Haeng RanKim, Se-JaeKim, Yuri
Ewha Authors
김유리
SCOPUS Author ID
김유리scopus
Issue Date
2015
Journal Title
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN
1422-0067JCR Link
Citation
vol. 16, no. 5, pp. 9976 - 9997
Keywords
Sasa quelpaertensis Nakai leaf extractcolon cancercancer stem cellsself-renewaldifferentiation
Publisher
MDPI AG
Indexed
SCIE; SCOPUS WOS scopus
Abstract
A rare subpopulation of cancer cells, termed cancer stem cells (CSCs), may be responsible for tumor relapse and resistance to conventional chemotherapy. The development of a non-toxic, natural treatment for the elimination of CSCs is considered a strategy for cancer treatment with minimal side effects. In the present study, the potential for Sasa quelpaertensis leaf extract (SQE) and its two bioactive compounds, tricin and p-coumaric acid, to exert anti-CSC effects by suppressing cancer stemness characteristics were evaluated in colon cancer cells. CD133(+)CD44(+) cells were isolated from HT29 and HCT116 cell lines using flow-activated cell sorting (FACs). SQE treatment was found to significantly suppress the self-renewal capacity of both cell lines. SQE treatment was also associated with the down-regulation of -catenin and phosphorylated GSK3, while significantly enhancing cell differentiation by up-regulating CK20 expression and blocking the expression of several stem cell markers, including DLK1, Notch1, and Sox-2. In vivo, SQE supplementation suppressed tumor growth in a xenograft model by down-regulating stem cell markers and -catenin as well as HIF-1 signaling. Compared with two bioactive compounds of SQE, SQE exhibited the most effective anti-CSC properties. Taken together, these results provide evidence that SQE inhibits colon cancer by regulating the characteristics of CSCs.
DOI
10.3390/ijms16059976
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신산업융합대학 > 식품영양학과 > Journal papers
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