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Osteogenic priming of mesenchymal stem cells by chondrocyte-conditioned factors and mineralized matrix

Title
Osteogenic priming of mesenchymal stem cells by chondrocyte-conditioned factors and mineralized matrix
Authors
Ro, HyunukPark, JunghaYang, KisukKim, JiyongYim, Hyun-GuJung, GiyoungLee, HyukjinCho, Seung-WooHwang, Nathaniel S.
Ewha Authors
이혁진
SCOPUS Author ID
이혁진scopus
Issue Date
2015
Journal Title
CELL AND TISSUE RESEARCH
ISSN
0302-766XJCR Link1432-0878JCR Link
Citation
vol. 362, no. 1, pp. 115 - 126
Keywords
Osteogenic differentiationMineralized scaffoldsSimulated body fluidsTissue engineeringStem cellsConditioned mediumIn vivo implantationMouse (BALB/c)
Publisher
SPRINGER
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Transient cartilage and a mineralizing microenvironment play pivotal roles in mesenchymal cell ossification during bone formation. In order to recreate these microenvironmental cues, C3H10T1/2 murine mesenchymal stem cells (MSCs) were exposed to chondrocyte-conditioned medium (CM) and seeded onto three-dimensional mineralized scaffolds for bone regeneration. Expansion of C3H10T1/2 cells with CM resulted in enhanced expression levels of chondrogenic markers such as aggrecan, type II collagen, type X collagen, and Sox9, rather than of osteogenic genes. Interestingly, CM expansion led to reduced expression levels of osteogenic genes such as alkaline phosphatase (ALP), type I collagen, osteocalcin, and Runx2. However, CM-expanded C3H10T1/2 cells showed enhanced osteogenic differentiation as indicated by increased ALP and Alizarin Red S staining upon osteogenic factor exposure. In vivo, CM-expanded C3H10T1/2 mesenchymal cells were seeded onto mineralized scaffolds (fabricated with polydopamine and coated with simulated body fluids) and implanted into critical-sized calvarial-defect mouse models. After 8 weeks of implantation, mouse skulls were collected, and bone tissue regeneration was evaluated by micro-computed tumography and Masson's trichrome staining. In accordance with the in vitro analysis, CM-expanded C3H10T1/2 cells gave enhanced bone mineral deposition. Thus, chondrocyte-conditioned factors and a mineralized microenvironment stimulate the bone formation of MSCs.
DOI
10.1007/s00441-015-2195-7
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약학대학 > 약학과 > Journal papers
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