Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이혁진 | * |
dc.date.accessioned | 2016-08-27T04:08:32Z | - |
dc.date.available | 2016-08-27T04:08:32Z | - |
dc.date.issued | 2015 | * |
dc.identifier.issn | 0302-766X | * |
dc.identifier.issn | 1432-0878 | * |
dc.identifier.other | OAK-14986 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/217237 | - |
dc.description.abstract | Transient cartilage and a mineralizing microenvironment play pivotal roles in mesenchymal cell ossification during bone formation. In order to recreate these microenvironmental cues, C3H10T1/2 murine mesenchymal stem cells (MSCs) were exposed to chondrocyte-conditioned medium (CM) and seeded onto three-dimensional mineralized scaffolds for bone regeneration. Expansion of C3H10T1/2 cells with CM resulted in enhanced expression levels of chondrogenic markers such as aggrecan, type II collagen, type X collagen, and Sox9, rather than of osteogenic genes. Interestingly, CM expansion led to reduced expression levels of osteogenic genes such as alkaline phosphatase (ALP), type I collagen, osteocalcin, and Runx2. However, CM-expanded C3H10T1/2 cells showed enhanced osteogenic differentiation as indicated by increased ALP and Alizarin Red S staining upon osteogenic factor exposure. In vivo, CM-expanded C3H10T1/2 mesenchymal cells were seeded onto mineralized scaffolds (fabricated with polydopamine and coated with simulated body fluids) and implanted into critical-sized calvarial-defect mouse models. After 8 weeks of implantation, mouse skulls were collected, and bone tissue regeneration was evaluated by micro-computed tumography and Masson's trichrome staining. In accordance with the in vitro analysis, CM-expanded C3H10T1/2 cells gave enhanced bone mineral deposition. Thus, chondrocyte-conditioned factors and a mineralized microenvironment stimulate the bone formation of MSCs. | * |
dc.language | English | * |
dc.publisher | SPRINGER | * |
dc.subject | Osteogenic differentiation | * |
dc.subject | Mineralized scaffolds | * |
dc.subject | Simulated body fluids | * |
dc.subject | Tissue engineering | * |
dc.subject | Stem cells | * |
dc.subject | Conditioned medium | * |
dc.subject | In vivo implantation | * |
dc.subject | Mouse (BALB/c) | * |
dc.title | Osteogenic priming of mesenchymal stem cells by chondrocyte-conditioned factors and mineralized matrix | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 362 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 115 | * |
dc.relation.lastpage | 126 | * |
dc.relation.journaltitle | CELL AND TISSUE RESEARCH | * |
dc.identifier.doi | 10.1007/s00441-015-2195-7 | * |
dc.identifier.wosid | WOS:000361470000010 | * |
dc.identifier.scopusid | 2-s2.0-84941877298 | * |
dc.author.google | Ro, Hyunuk | * |
dc.author.google | Park, Jungha | * |
dc.author.google | Yang, Kisuk | * |
dc.author.google | Kim, Jiyong | * |
dc.author.google | Yim, Hyun-Gu | * |
dc.author.google | Jung, Giyoung | * |
dc.author.google | Lee, Hyukjin | * |
dc.author.google | Cho, Seung-Woo | * |
dc.author.google | Hwang, Nathaniel S. | * |
dc.contributor.scopusid | 이혁진(55233457200) | * |
dc.date.modifydate | 20240220111730 | * |