View : 54 Download: 0

Splice variant of the C2H2-type zinc finger protein, ZNF268s, regulates NF-kappa B activation by TNF-alpha

Title
Splice variant of the C2H2-type zinc finger protein, ZNF268s, regulates NF-kappa B activation by TNF-alpha
Authors
Chun, Jung NyeoSong, In SungKang, Dong-HoonSong, Hye JinKim, Hye InSuhl, Ja WonLee, Kong JuKim, JaesangKang, Sang Won
Ewha Authors
이공주강상원김재상강동훈
SCOPUS Author ID
이공주scopus; 강상원scopus; 김재상scopus; 강동훈scopus
Issue Date
2008
Journal Title
MOLECULES AND CELLS
ISSN
1016-8478JCR Link
Citation
vol. 26, no. 2, pp. 175 - 180
Keywords
C2H2-type zinc finger proteinI kappa B kinaseKRABNF-kappa BTNF-alphaZNF268
Publisher
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
I kappa B kinase (IKK), the pivotal kinase in signal-dependent activation of nuclear factor-kappa B (NF-kappa B), is composed of multiple protein components, including IKK alpha/beta/gamma core subunits. To investigate the regulation of the IKK complex, we immunoaffinity purified the IKK complex, and by MALDI-TOF mass spectrometry identified a splice variant of zinc finger protein 268 (ZNF268) as a novel IKK-interacting protein. Both the full-length and the spliced form of the ZNF268 protein were detected in a variety of mammalian tissues and cell lines. The genes were cloned and expressed by in vitro transcription/translation. Several deletion derivatives, such as KRAB domain (KRAB) on its own, the KRAB/spacer/4-zinc fingers (zF4), and the spacer/4-zinc fingers (zS4), were ectopically expressed in mammalian cells and exhibited had different subcellular locations. The KRAB-containing mutants were restricted to the nucleus, while zS4 was localized in the cytosol. TNF-alpha-induced NF-kappa B activation was examined using these mutants and only zS4 was found to stimulate activation. Collectively, the results indicate that a spliced form of ZNF268 lacking the KRAB domain is located in the cytosol, where it seems to play a role in TNF-alpha-induced NF-kappa B activation by interacting with the IKK complex.
Appears in Collections:
약학대학 > 약학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE