Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이공주 | * |
dc.contributor.author | 강상원 | * |
dc.contributor.author | 김재상 | * |
dc.contributor.author | 강동훈 | * |
dc.date.accessioned | 2016-08-27T02:08:53Z | - |
dc.date.available | 2016-08-27T02:08:53Z | - |
dc.date.issued | 2008 | * |
dc.identifier.issn | 1016-8478 | * |
dc.identifier.other | OAK-5041 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/216270 | - |
dc.description.abstract | I kappa B kinase (IKK), the pivotal kinase in signal-dependent activation of nuclear factor-kappa B (NF-kappa B), is composed of multiple protein components, including IKK alpha/beta/gamma core subunits. To investigate the regulation of the IKK complex, we immunoaffinity purified the IKK complex, and by MALDI-TOF mass spectrometry identified a splice variant of zinc finger protein 268 (ZNF268) as a novel IKK-interacting protein. Both the full-length and the spliced form of the ZNF268 protein were detected in a variety of mammalian tissues and cell lines. The genes were cloned and expressed by in vitro transcription/translation. Several deletion derivatives, such as KRAB domain (KRAB) on its own, the KRAB/spacer/4-zinc fingers (zF4), and the spacer/4-zinc fingers (zS4), were ectopically expressed in mammalian cells and exhibited had different subcellular locations. The KRAB-containing mutants were restricted to the nucleus, while zS4 was localized in the cytosol. TNF-alpha-induced NF-kappa B activation was examined using these mutants and only zS4 was found to stimulate activation. Collectively, the results indicate that a spliced form of ZNF268 lacking the KRAB domain is located in the cytosol, where it seems to play a role in TNF-alpha-induced NF-kappa B activation by interacting with the IKK complex. | * |
dc.language | English | * |
dc.publisher | KOREAN SOC MOLECULAR & | * |
dc.publisher | CELLULAR BIOLOGY | * |
dc.subject | C2H2-type zinc finger protein | * |
dc.subject | I kappa B kinase | * |
dc.subject | KRAB | * |
dc.subject | NF-kappa B | * |
dc.subject | TNF-alpha | * |
dc.subject | ZNF268 | * |
dc.title | Splice variant of the C2H2-type zinc finger protein, ZNF268s, regulates NF-kappa B activation by TNF-alpha | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 26 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 175 | * |
dc.relation.lastpage | 180 | * |
dc.relation.journaltitle | MOLECULES AND CELLS | * |
dc.identifier.wosid | WOS:000258949800011 | * |
dc.identifier.scopusid | 2-s2.0-53749087716 | * |
dc.author.google | Chun, Jung Nyeo | * |
dc.author.google | Song, In Sung | * |
dc.author.google | Kang, Dong-Hoon | * |
dc.author.google | Song, Hye Jin | * |
dc.author.google | Kim, Hye In | * |
dc.author.google | Suhl, Ja Won | * |
dc.author.google | Lee, Kong Ju | * |
dc.author.google | Kim, Jaesang | * |
dc.author.google | Kang, Sang Won | * |
dc.contributor.scopusid | 이공주(7501497635;57191532162) | * |
dc.contributor.scopusid | 강상원(55731433900) | * |
dc.contributor.scopusid | 김재상(8643335800) | * |
dc.contributor.scopusid | 강동훈(57033374200) | * |
dc.date.modifydate | 20240429130259 | * |