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Effects of a tetramethoxyhydroxyflavone on the expression of inflammatory mediators in LPS-treated human synovial fibroblast and macrophage cells

Title
Effects of a tetramethoxyhydroxyflavone on the expression of inflammatory mediators in LPS-treated human synovial fibroblast and macrophage cells
Authors
Doyoung, YoonCho, MinchullKim, Jung-HeeKim, Eun-JinKang, Jeong-WooSeo, Eun-HeeShim, Jung-HyunKim, Soo-HyunLee, Hee-GuOh, Goo-TaegHong, Jin-TaePark, JoowonKim, Jong-Wan
Ewha Authors
오구택
SCOPUS Author ID
오구택scopus
Issue Date
2008
Journal Title
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
ISSN
1017-7825JCR Link
Citation
vol. 18, no. 4, pp. 686 - 694
Keywords
anti-inflammatory agentflavonesynovial fibroblastsinflammatory mediatorsproinflammatory cytokines
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
The inhibitory effects of 5,6,3',5'-tetramethoxy 7,4'-hydroxyflavone (labeled as p7F) were elucidated on the productions of proinflammatory cytokines as well as inflammatory mediators in human synovial fibroblasts and macrophage cells. p7F inhibited IL-1 beta or TNF-alpha induced expressions of inflammatory mediators (ICAM-1, COX-2, and iNOS). p7F also inhibited LPS-induced productions of nitric oxide and prostaglandin E, in RAW 264.7 cells. In order to investigate whether p7F would inhibit IL-1 signaling, p7F was added to the D10S Th2 cell fine (which is responsive to only IL-1 beta and thus proliferates), revealing that p7F inhibited IL-1 beta-induced proliferation of D10S Th2 cells in a dose-response manner. A flow cytometric analysis revealed that p7F reduced the intracellular level of free radical oxygen species in RAW 264.7 cells treated with hydrogen peroxide. p7F inhibited 1 kappa B degradation and NF-kappa B activation in macrophage cells treated with LPS, supporting that p7F could inhibit signaling mediated via toll-like receptor. Taken together, p7F has inhibitory effects on LPS-induced productions of inflammatory mediators on human synovial fibroblasts and macrophage cells and thus has the potential to be an anti-inflammatory agent for inhibiting inflammatory responses.
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자연과학대학 > 생명과학전공 > Journal papers
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