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dc.contributor.author강순자-
dc.date.accessioned2016-08-27T02:08:39Z-
dc.date.available2016-08-27T02:08:39Z-
dc.date.issued2007-
dc.identifier.issn1226-3613-
dc.identifier.otherOAK-4102-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/216112-
dc.description.abstractDopamine (DA) is an oxidant that may contribute to the degeneration of dopaminergic neurons. The present study demonstrates that DA-induced cytotoxicity in human-derived neurotypic cells, SH-SY5Y, is prevented by resveratrol, one of the major antioxidative constituents found in the skin of grapes. SH-SY5Y cells, a neuroblastoma cell line, treated with DA at 300 and 500 mu M for 24 h underwent apoptotic death as determined by characteristic morphological features, including nuclear condensation, and loss of mitochondrial membrane potential (MMP). Flow cytometric analysis using Annexin V showed that DA can induce significant and severe apoptosis. Exposure to resveratrol (5 mu M) for 1 h prior to the DA treatment attenuated DA-induced cytotoxicity, and rescued the loss of MMP. To investigate the apoptotic signaling pathways relevant to the restoration of DA-induced apoptosis by resveratrol, we carried out quantitative analysis of Bcl-2, caspase-3, and cleaved poly ADP-ribose polymerase (PARP) by immunoblot analysis. Resveratrol pretreatment led to a decrease in cleavage of PARP, an increase in the Bcl-2 protein, and activation of caspase-3. These results suggest that DA may be a potential oxidant of neuronal cells at biologically relevant concentrations. Resveratrol may protect SH-SY5Y cells against this cytotoxicity, reducing intracellular oxidative stress through canonical signal pathways of apoptosis and may be of biological importance in the prevention of a dopaminergic neurodegenerative disorder such as Parkin son disease.-
dc.languageEnglish-
dc.publisherKOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY-
dc.subjectantioxidant-
dc.subjectapoptosis-
dc.subjectdopamine-
dc.subjectneuroblastoma-
dc.subjectneurodegenerative diseases-
dc.subjectresveratrol-
dc.titleResveratrol protects SH-SY5Y neuroblastoma cells from apoptosis induced by dopamine-
dc.typeArticle-
dc.relation.issue3-
dc.relation.volume39-
dc.relation.indexSCI-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.indexKCI-
dc.relation.startpage376-
dc.relation.lastpage384-
dc.relation.journaltitleEXPERIMENTAL AND MOLECULAR MEDICINE-
dc.identifier.wosidWOS:000247798800015-
dc.identifier.scopusid2-s2.0-34447136104-
dc.author.googleLee, Mi Kyung-
dc.author.googleKang, Soon Ja-
dc.author.googlePoncz, Mortimer-
dc.author.googleSong, Ki-Joon-
dc.author.googlePark, Kwang Sook-
dc.contributor.scopusid강순자(36927151700;36479176200)-
dc.date.modifydate20180104081001-


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