Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 강순자 | - |
dc.date.accessioned | 2016-08-27T02:08:39Z | - |
dc.date.available | 2016-08-27T02:08:39Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.other | OAK-4102 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/216112 | - |
dc.description.abstract | Dopamine (DA) is an oxidant that may contribute to the degeneration of dopaminergic neurons. The present study demonstrates that DA-induced cytotoxicity in human-derived neurotypic cells, SH-SY5Y, is prevented by resveratrol, one of the major antioxidative constituents found in the skin of grapes. SH-SY5Y cells, a neuroblastoma cell line, treated with DA at 300 and 500 mu M for 24 h underwent apoptotic death as determined by characteristic morphological features, including nuclear condensation, and loss of mitochondrial membrane potential (MMP). Flow cytometric analysis using Annexin V showed that DA can induce significant and severe apoptosis. Exposure to resveratrol (5 mu M) for 1 h prior to the DA treatment attenuated DA-induced cytotoxicity, and rescued the loss of MMP. To investigate the apoptotic signaling pathways relevant to the restoration of DA-induced apoptosis by resveratrol, we carried out quantitative analysis of Bcl-2, caspase-3, and cleaved poly ADP-ribose polymerase (PARP) by immunoblot analysis. Resveratrol pretreatment led to a decrease in cleavage of PARP, an increase in the Bcl-2 protein, and activation of caspase-3. These results suggest that DA may be a potential oxidant of neuronal cells at biologically relevant concentrations. Resveratrol may protect SH-SY5Y cells against this cytotoxicity, reducing intracellular oxidative stress through canonical signal pathways of apoptosis and may be of biological importance in the prevention of a dopaminergic neurodegenerative disorder such as Parkin son disease. | - |
dc.language | English | - |
dc.publisher | KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY | - |
dc.subject | antioxidant | - |
dc.subject | apoptosis | - |
dc.subject | dopamine | - |
dc.subject | neuroblastoma | - |
dc.subject | neurodegenerative diseases | - |
dc.subject | resveratrol | - |
dc.title | Resveratrol protects SH-SY5Y neuroblastoma cells from apoptosis induced by dopamine | - |
dc.type | Article | - |
dc.relation.issue | 3 | - |
dc.relation.volume | 39 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.index | KCI | - |
dc.relation.startpage | 376 | - |
dc.relation.lastpage | 384 | - |
dc.relation.journaltitle | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.identifier.wosid | WOS:000247798800015 | - |
dc.identifier.scopusid | 2-s2.0-34447136104 | - |
dc.author.google | Lee, Mi Kyung | - |
dc.author.google | Kang, Soon Ja | - |
dc.author.google | Poncz, Mortimer | - |
dc.author.google | Song, Ki-Joon | - |
dc.author.google | Park, Kwang Sook | - |
dc.contributor.scopusid | 강순자(36927151700;36479176200) | - |
dc.date.modifydate | 20180104081001 | - |