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Identification of a domain in yeast chitin synthase 3 interacting with chitin synthase 4 by two-hybrid analysis
- Identification of a domain in yeast chitin synthase 3 interacting with chitin synthase 4 by two-hybrid analysis
- Park, YS; Choi, SJ; Park, NH; Kim, CH; Kim, SU; Choi, W
- Ewha Authors
- SCOPUS Author ID
- Issue Date
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- vol. 12, no. 6, pp. 943 - 949
- chitin synthase 3; two-hybrid analysis; Saccharomyces cerevisiae; ScCHS4
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- SCIE; SCOPUS; KCI
- It has been proposed that chitin synthase 3 (CHS3)-mediated chitin synthesis during the vegetative cell cycle is regulated by chitin synthase 4 (CHS4) of Saccharomyces cerevisiae. To investigate direct protein-protein interaction between the coding products of these two genes, a domain of Chs3p that is responsible for interaction with Chs4p was identified, using the yeast two-hybrid system. This domain of 54 amino acids, termed MIRC3-4 (Maximum Interacting Region of Chs3p with Chs4p), is well conserved among CHS3 homologs of various fungi. Some mutations in MIRC3-4 resulted in a decrease in the enzymatic activity and chitin contents. Chs3p carrying those mutations exhibited weak interactions with Chs4p, when assayed by the yeast two-hybrid system. Surprisingly, all the mutants were sensitive to Calcofluor regardless of changes in enzymatic activities or chitin contents. This report deals with a core region in MIRC3-4 that affects the interaction with Chs4p.
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