Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 최원자 | - |
dc.date.accessioned | 2016-08-27T02:08:45Z | - |
dc.date.available | 2016-08-27T02:08:45Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.other | OAK-1290 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/215555 | - |
dc.description.abstract | It has been proposed that chitin synthase 3 (CHS3)-mediated chitin synthesis during the vegetative cell cycle is regulated by chitin synthase 4 (CHS4) of Saccharomyces cerevisiae. To investigate direct protein-protein interaction between the coding products of these two genes, a domain of Chs3p that is responsible for interaction with Chs4p was identified, using the yeast two-hybrid system. This domain of 54 amino acids, termed MIRC3-4 (Maximum Interacting Region of Chs3p with Chs4p), is well conserved among CHS3 homologs of various fungi. Some mutations in MIRC3-4 resulted in a decrease in the enzymatic activity and chitin contents. Chs3p carrying those mutations exhibited weak interactions with Chs4p, when assayed by the yeast two-hybrid system. Surprisingly, all the mutants were sensitive to Calcofluor regardless of changes in enzymatic activities or chitin contents. This report deals with a core region in MIRC3-4 that affects the interaction with Chs4p. | - |
dc.language | English | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & | - |
dc.publisher | BIOTECHNOLOGY | - |
dc.subject | chitin synthase 3 | - |
dc.subject | two-hybrid analysis | - |
dc.subject | Saccharomyces cerevisiae | - |
dc.subject | ScCHS4 | - |
dc.title | Identification of a domain in yeast chitin synthase 3 interacting with chitin synthase 4 by two-hybrid analysis | - |
dc.type | Article | - |
dc.relation.issue | 6 | - |
dc.relation.volume | 12 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.index | KCI | - |
dc.relation.startpage | 943 | - |
dc.relation.lastpage | 949 | - |
dc.relation.journaltitle | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.identifier.wosid | WOS:000180146800013 | - |
dc.identifier.scopusid | 2-s2.0-0037003103 | - |
dc.author.google | Park, YS | - |
dc.author.google | Choi, SJ | - |
dc.author.google | Park, NH | - |
dc.author.google | Kim, CH | - |
dc.author.google | Kim, SU | - |
dc.author.google | Choi, W | - |
dc.contributor.scopusid | 최원자(7402515987) | - |
dc.date.modifydate | 20230210130537 | - |