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식이내 카드뮴과 단백질 수준이 흰쥐의 체내 단백질 대사 및 카드뮴 중독에 미치는 영향

Title
식이내 카드뮴과 단백질 수준이 흰쥐의 체내 단백질 대사 및 카드뮴 중독에 미치는 영향
Other Titles
Effects of cadmium and dietary protein levels on the body protein metabolism and cadmium toxicity in growing rats
Authors
李惠寧
Issue Date
1988
Department/Major
대학원 식품영양학과
Keywords
식이카드뮴단백질카드뮴중독단백질대사
Publisher
이화여자대학교 대학원
Degree
Master
Advisors
金美經
Abstract
본 연구는 Cadmium중독이 성장기 흰쥐의 성장 및 체내 단백질 대사에 미치는 영향과 식이 단백질 수준이 Cadmium 중독에 미치는 영향을 함께 알아보고자 하였다. 평균체중이 112.5±2.1g Sprague Dawley종 숫컷 흰쥐 48마리를 식이 단백질 수준을 7%, 15%, 40%로 달리 한후 Cd공급군(CdCl_(2)200ppm)과 비공급군으로 나누어 30일동안 사육하였고, Cd balance study를 위해 실험 종료 전 5일 동안 0.1㎖의 CdCl_(2)용액 (CdCl_(2)1㎎)을 tube feeding으로 공급하고 3일동안 뇨와 변을 채취하였다. 그 결과는 다음과 같이 요약된다. 식이 섭취량 및 체중 증가량은 Cd공급군들이 비공급군들에 비해 유의적으로 낮았고, Cd공급군들 중 저단백질군에서 가장 감소가 심하였다. 간과 신장의 무게, 혀의 길이와 무게는 Cd공급시 모두 감소되었으나, 식이 단백질 수준이 높을수록 증가되었다. 대퇴골의 회분 함량과 Ca함량은 Cd공급군들이 비공급군들보다 낮았으나, 식이 단백질 수준에 따른 뚜렷한 영향은 나타나지 않았다. Hemoglobin 함량과 Hematocrit값은 Cd공급군들이 비공급군들 보다 낮았으나, 식이 단백질 수준에 의한 영향은 없었다. 혈청 및 간의 총 단백질함량은 Cd공급군들이 비공급군들 보다 낮았으며, 식이 단백질 수준이 높을수록 증가되었다. 뇨와 변을 통한 질소배설량은 Cd공급군들이 비공급군들 보다 낮았으며, 식이 단백질 수준이 높을수륵 증가하였다. 체내 질소 흡수율은 저단백질군에서만 Cd섭취에 의해 유의적으로 감소되었고, 식이 단백질 수준이 높을수록 증가되었다. 혈액, 간, 신장, 뼈의 Cd함량은 식이 단백질 수준이 높을수록 감소되는 경향을 보였다. 특히, 신장의 경우 Cd축적이 가장 높았고, 표준단백질군과 고단백질군이 저단백질군에 비해 유의적으로 낮았다. 뇨와 변을 통한 Cd배설은 식이 단백질 수준이 높을수록 증가하는 경향을 보여 고단백질군에서 가장 높았으며, 따라서 체내 Cd흡수율과 보유율이 고단백질군에서 가장 낮았다. 이상의 결과로 볼때, Cd섭취시 식이 섭취량 감소등으로 성장이 부진하였으며, 혈청 및 간의 단백질 함량 저하등 체내 단백질 대사에 영향을 미쳤다. 그리고 이러한 Cd중독의 영향은 식이 단백질 수준이 높을수록 완화되었는데, 이는 단백질에 의해 Cd흡수가 억제되고 뇨를 통한 배설이 증가됨에 따라 조직내 Cd축적 및 체내 Cd보유량이 감소되었기 때문으로 생각된다.;This study was performed to investigate the effects of Cadmium(Cd) and different levels of dietary protein (7, 15 and 40% casein diets) on the growth, body protein metabolism and Cd toxicity in growing rats. Forty eight male rats of Sprague-Dawley strain weithing 112.5±2.lg were blocked into 6 groups according to body weight. Protein were given at the levels of 7, 15 and 40% of total caloric intake and Cd(200ppm) were either added or not. The results obtained were summerized as follow: 1) Both of food intake and weight gain in Cd-added groups were significantly lower than those in Cd-free groups. Especially. they were decreased severly in Cd-added low protein diet group. On the other hand, F. E. R. was increased with increasing dietary protein level, P. E. R. was decreased with increasing dietary protein level. 2) The liver weight, kidney weight, both of the length and weight of femur and tibia were decreased in Cd-added groups. but, all of them were increased with increasing dietary protein level. Both of the ash content and calcium content of femur in Cd-added groups were lower than those in Cd-free groups, but there were no clear differences among groups with different levels of protein. 3) Hemoglobin content and hematocrit value in Cd-added groups were lower than those in Cd-free groups, but there no differences among groups with different levels of protein. 4) Serum total protein showed no significant difference with Cd addition. However, serum total protein levels in low protein diet groups were significantly lower than those of any other groups. Both of the content of total protein in each gram of liver and total liver in Cd-added groups were lower than those in Cd-free groups. They did not show significant difference, but showed increasing tendency with increasing dietary protein level. 5) Both of daily urinary and fecal nitrogen excretion in Cd-added groups were lower than those in Cd-free groups. And they were increased with increasing dietary protein level. Body nitrogen absorption rate was significantly decreased only in Cd-added low protein diet group, it was increased with increasing dietary protein level. 6) The Cd concentration levels of blood, liver, kidney, bone were tend to be decreased with increasing dietary protein level. Especially, the Cd concentration level of kidney was higher in Cd-added low protein diet group than those of any other Cd-added groups. 7) The amount of daily urinary and fecal Cd excretion were tend to be increased with increasing dietary protein led. And. Cd-added high protein diet group showed the highest Cd excretion among all of the Cd-added groups. Therefore, body Cd absorption rate and body Cd retention in Cd-added high protein diet group were the lowest among all of the Cd-added groups.
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