성장초기 인삼의 투여가 흰쥐의 신체대사에 미치는 영향

Abstract

본 연구는 성장초기에 투여한 인삼이 성장후기에 성장제한식이 및 고콜레스테롤식이로 사육했을때의 흰쥐의 신체대사에 미치는 영향을 살펴보았다. 이유직후의 평균체중이 60.3±1.7g인 Sprague-Dawley종 수컷 흰쥐 56마리를 표준식이를 섭취하는 인삼무첨가군과 표준식이와 함께 인삼분말을 체중 kg당 500mg수준으로 섭취하는 인삼첨가군으로 각 군당 28마리씩 나누어 6주간 사육한 뒤, 각 실험군을 3가지 실험식이군으로 나누어 고콜레스테롤식이, 성장제한식이 및 표준식이로 다시 6주동안 사육함으로써 성장초기에 섭취한 인삼이 성장후기의 식이변화에 의한 결과에 어떤 영향을 주었는지를 살펴보고자 각 실험군에서 식이섭취량과 체중의 변화를 측정하였고 성장제한식이군에서는 뇨중 질소함량 및 creatinine 함량과 골격근육의 무게, 골격근육의 질소함량 및 지방함량을 측정하였으며, 고콜레스테롤식이군에서는 혈청 총 콜레스테롤함량 및 총 지방함량을 측정하였다. 식이섭취취량은 실험 전기간동안, 인삼첨가나 식이의 종류에 관계없이 모든 실험군에서 유의적인 차이가 없었다. 체중의 변화는 인삼첨가군과 인삼무첨가군 간에 유의적인 차이가 없었고, 성장제한식이군이 표준식이군과 고콜레스테롤식이군보다 유의적으로 낮았다. 뇨중 creatinine함량은 인삼무첨가군중 성장제한식이군과 표준식이군 사이에 유의적인 차이가 없었고, 인삼첨가군중 성장제한식이군은 표준식이군보다 유의적으로 낮았다. creatinine coefficient는 표준식이군 및 성장제한식이군 모두 유의적인 차이가 없었고, 같은 식이군내에서 인삼첨가군이 인삼무첨가군보다 높은 경향이었으나 유의적인 차이는 아니었다. 뇨의 총질소함량은 표준식이군이 성장제한식이군보다 유의적으로 높았고, 표준식이군중 인삼첨가군이 인삼무첨가군보다 유의적으로 높았으며, 성장제한식이군내에서는 인삼첨가군과 인삼무첨가군간에 유의적인 차이가 없었다. 골격근육무게는 실험 첫 6주에서 인삼첨가군과 인삼무첨가군사이에 유의적인 차이가 없었으며, 식이를 변화시킨 실험후기 6주에서 성장제한식이군이 표준식이군보다 유의적으로 낮았다. 골격근육의 총질소함량은 인삼첨가군과 인삼무첨가군사이에 유의적인 차이가 없었고, 인삼무첨가군중 성장제한식이군이 표준식이군보다 유의적으로 낮았으며, 인삼첨가군에선 성장제한식이군과 표준식이군간에 유의적인 차이가 없었다. 골격근육의 총지방함량은 인삼무첨가군과 인삼첨가군 및 성장제한 식이군과 표준식이군에서 모두 유의적인 차이가 없었다. 혈청 총콜레스테롤함량은 인삼첨가군과 인삼무첨가군사이에 유의적인 차이가 없었고, 고콜레스테롤식이군이 표준식이군보다 유의적으로 높았다. 혈청 총 지방함량은 인삼무첨가군중 고콜레스테롤식이군이 인삼첨가군중 표준식이군보다 유의적으로 높았고, 그외이 실험군들에서는 유의적인 차이가 없었다. 이상의 결과로 볼 때, 성장초기에 섭취한 인삼이 그 후의 식이변화로 인한 결과에 어떤 유의적인 영향도 미치지 않은 것으로 나타났으므로 인삼의 투여용량과 투여시기 등에 관한 연구가 보다 더 요구되는 바이다.;This study was designed to observe the effect of diet supplemented with Ginseng powder in early period of growth on the body metabolism in rats fed low protein diet or high cholesterol diet in later period of growth. Fifty six Sprague - Dawley male rats weighing 60.3±1.7g were divided into two groups during first six weeks of experimental periods, one for control group fed standard diet, the other for Ginseng group fed standard diet supplemented with Ginseng powder. For six weeks, Ginseng group received every day 500mg/kg body weight of Ginseng powder by seperated feeding method. Seven rats of each group were sacrificed at the sixth week of experimental periods and the rest of each group were subdivided into three different groups, seven rats each, the first group were fed standard diet contained 18% casein, the second were fed growth retardation diet contained 3% casein, the third were fed high cholesterol diet contained 2% cholesterol per kg diet for last six weeks of experimental periods. At the end of experimental periods, all forty two rats were sacrificed. Following assessments were done in this experiment. The amounts of food intake, body weight were measured in all experimental groups. For the growth retardation group, skeletal muscles were weighed, total nitrogen and creatinine contents in urine, total nitrogen and total lipid contents in skeletal muscles were analyzed. For the high cholesterol diet group, serum total cholesterol and total lipid contents were measured. The result of this experiment were summarized as follows; Total iood intake through all experimental periods did not show statistical significance. Body weight gain also tended to be similar to the above. Growth retardation diet group was significantly lower body weight than the. other diet groups. Creatinine excretions in urine tended to be higher in standard diet group than in growth retardation diet group. Creatinine coefficient did not show significant differences among standard diet group and growth retardation diet group. Urinary nitrogen contents of the standard diet group were higher than those of the growth retardation diet group. The skeletal muscle weights showed the same tendency as body weight. There was no significant difference in all kinds (Anterior Tibialis, Extensor Digitorum Longus, Soleus, Plantaris, Gastrocnemius) of skeletal muscle weight between ginseng group and control group. After dietary changes for last six weeks of experimental periods, muscle weights in growth retardation diet group were less than those of standard diet group. There was no significant difference of nitrogen content in skeletal muscles due to the addition of Ginseng in the diet for the first six weeks of experimental periods. After twelve weeks, in the control groups, nitrogen content in muscles of growth retardation diet group was significantly less than that of standard diet group. In the ginseng groups, there was no significance in nitrogen content of muscles between growth retardation diet group and standard diet group. Total lipid content in five muscles did not show statistical significance in all experimental groups. But there was a tendency to be decreased in the growth retardation diet groups compared with the standard diet groups. Serum total cholesterol content showed no significance between ginseng group and control group for the first six weeks. After the last six weeks of experimental periods, it was significantly higher in high cholesterol diet group than in the standard diet group. In the groups of same diet, it showed increasing tendency in group fed diet supplemented with ginseng for the first six weeks compared with control group, but it did not show statistical significance. There was no significant difference of serum total lipid content due to the addition of ginseng or cholesterol in the diet, but, it showed increasing tendency in ginseng group compared with control group for the first six weeks, and after changing diet with high cholesterol diet, it showed statistical significance in all experimental groups. But there was a tendency 'to be decreased in the grouth retardation diet groups compared with the standard diet groups. Serum total cholesterol content showed no significance between ginseng group and control group for the first six weeks. After the last six weeks of experimental periods, it was significantly higher in high cholesterol diet group than in the standard diet group. In the groups of same diet, it showed increasing tendency in ginseng group compared with control group, but it did not show statistical significance. There was no significant difference of serum total lipid content due to the addition of ginseng or cholesterol in the diet. Considering the results obtained from this study, the body metabolism in rats fed high cholesterol or growth retardation diet in later growing periods were not significantly influenced by fed normal diet supplemented with ginseng in early growing periods.