밀감과피로부터 식이섬유와 bioflavonoid의 정제과정중 fenitrothion농약 잔류분의 제거

Abstract

본 연구에서는 밀감에 유기인계 농약인 fenitrothion 성분을 인위적으로 부착시킨 후, 밀감과피에서 식이섬유 및 bioflavonoid의 정제과정중 농약성분의 제거율을 실험하였다. 신선한 밀감과피로부터 정제된 총 식이섬유(total dietary fiber, TDF)와 불용성 식이섬유(insoluble dietary fiber, IDF) 및 수용성 식이섬유(soluble dietary fiber, SDF)의 수득율은 풍건물 기준으로 각각 17.4%, 13.1%, 1.7%로 나타났다. 밀감과피에 0.5 ppm 및 13 ppm의 fenitrothion 성분을 부착시킨 후, 과피의 마쇄, 효소반응, 에탄올 침전 및 여과, 아세톤 세척, 풍건등을 거쳐 식이섬유를 정제하였을때, fenitrothion 성분의 제거율을 비교해 보았다. 고농도와 저농도로 오염된 밀감과피의 식이섬유 정제과정 동안에 fenitrothion 성분의 제거율을 살펴보면 각각 TDF에서 98.4%, 91.9%, IDF에서 99.7%, 97.1%, 그리고 SDF에서 100.0%, 99.6%로 나타났다. 고농도와 저농도로 fenitrothion이 오염된 밀감과피의 식이섬유 정제과정중 농약성분의 제거율은 비슷하였으나, 고농도로 오염된 밀감과피의 제거율이 약간 높게 나타났다. 밀감과피의 마쇄, 열수추출, 에탄올 침전, hexane 및 butanol 추출 등의 bioflavonoid 정제과정 동안에 fenitrothion 성분의 제거율은 intermediate extract에서 92.7%, bioflavonoid extract에서는 100.0%로 나타났다. 결론적으로 밀감과피에 잔류하는 fenitrothion 성분은 식이섬유와 bioflavonoid의 정제도중에 그의 대부분이 제거되는 것으로 나타났다. 그러므로 본 연구결과는 밀감과피로부터 식이섬유 및 bioflavonoid와 같은 기능성 물질을 정제하는데 있어 잔류농약 성분의 안전성에 대한 기초자료로 이용될 수 있다고 생각된다.;This study was undertaken in order to elucidate the elimination of fenitrothion residues during dietary fiber and bioflavonoid preparation from mandarin orange peels. The results are summarized as follows. Yields of total dietary fiber(TDF), insoluble dietary fiber(IDF) and soluble dietary fiber(SDF) were 17.4%, 13.1% and l.7%, respectively. When dietary fiber was preparated from contaminated mandarin orange peels at 13 ppm and 0.5 ppm concentrations, the procedures were homogenating, enzyme treatment, ethanol precipitating and filtering, acetone washing and air drying, etc. During preparations of dietary fiber from mandarin orange peels which were contaminated with high and low concentration fenitrothion, the removal rate from contaminated mandarin orange peels was 98.4% and 91.9% in TDF, 99.7% and 97.1% in IDF, 100.0% and 99.6% in SDF, respectively. When the removal rate from contaminated mandarin orange peels were compared for high and low concentration levels, both were similar but the removal rate of contaminated mandarin orange peels with high concentration was somewhat higher than contaminated mandarin orange peels with low concentration. When bioflavonoid was preparated from contaminated mandarin orange peels, the procedures were homogenating, soaking, ethanol precipitating, hexane and butanol extraction, etc. The removal rate from contaminated mandarin orange peels was 92.7% in intermediate extract and 100.0% in bioflavonoid extract. In conclusion, fenitrothion residue in mandarin orange peels was removed mostly during dietary fiber and bioflavonoid preparations, respectively. When dietary fiber and bioflavonoid were prepared from mandarin orange peels, the results can be applied to safety measures of pesticide residue.