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A novel chalcone derivative exerts anticancer effects by promoting apoptotic cell death of human pancreatic cancer cells
- Title
- A novel chalcone derivative exerts anticancer effects by promoting apoptotic cell death of human pancreatic cancer cells
- Authors
- Baek; Suji; Nah; Sanghee; Park; Joo Yeon; Lee; Sang Ju; Kang; Yong Gil; Kwon; Seung Hae; Oh; Seung Jun; Kang Pa; Moon; Byung Seok
- Ewha Authors
- 문병석
- SCOPUS Author ID
- 문병석
![scopus](/images/layout/icon2.png)
![scopus](/images/layout/icon2.png)
- Issue Date
- 2023
- Journal Title
- Bioorganic and Medicinal Chemistry
- ISSN
- 0968-0896
- Citation
- Bioorganic and Medicinal Chemistry vol. 93
- Keywords
- Apoptosis; Chalcone; Lipid accumulation; Pancreatic cancer; Stimulated Raman scattering microscopy
- Publisher
- Elsevier Ltd
- Indexed
- SCIE; SCOPUS
![scopus](/images/layout/scopus2.gif)
- Document Type
- Article
- Abstract
- Aggressive pancreatic cancer is typically treated using chemotherapeutics to reduce the tumor pre-operatively and prevent metastasis post-operatively, as well as surgical approaches. In the present study, we synthesized a hydroxyl group-introduced chalcone derivative (1, IC50 = 32.1 μM) and investigated its potential as an anticancer drug candidate by evaluating its apoptosis-promoting effects on BXPC-3 cancer cells. The viability of BXPC-3 cells treated with 1 was measured using the water-soluble tetrazolium 1 reagent. BXPC-3 cells induced by 1 were stained with diverse probes or antibodies, such as ethidium homodimer-1, Hoechst, anti-Ki67, and MitoTracker. Protein expression was measured using an immunoblotting assay, and mRNA expression was determined using real-time polymerase chain reaction. Apoptotic molecular features, such as lipid accumulation and protein degradation, were monitored directly using stimulated Raman scattering microspectroscopy. Through incubation time- and concentration-dependent studies of 1, we found that it significantly reduced the proliferation and increased the number of apoptotic BXPC-3 cells. Compound 1 induced mitochondrial dysfunction, phosphorylation of p38, and caspase 3 cleavage. These results indicate that 1 is a potential therapeutic agent for pancreatic cancer, providing valuable insights into the development of new anticancer drug candidates. © 2023 Elsevier Ltd
- DOI
- 10.1016/j.bmc.2023.117458
- Appears in Collections:
- 의료원 > 의료원 > Journal papers
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