Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 문병석 | * |
dc.date.accessioned | 2024-01-31T16:31:25Z | - |
dc.date.available | 2024-01-31T16:31:25Z | - |
dc.date.issued | 2023 | * |
dc.identifier.issn | 0968-0896 | * |
dc.identifier.other | OAK-34029 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/266893 | - |
dc.description.abstract | Aggressive pancreatic cancer is typically treated using chemotherapeutics to reduce the tumor pre-operatively and prevent metastasis post-operatively, as well as surgical approaches. In the present study, we synthesized a hydroxyl group-introduced chalcone derivative (1, IC50 = 32.1 μM) and investigated its potential as an anticancer drug candidate by evaluating its apoptosis-promoting effects on BXPC-3 cancer cells. The viability of BXPC-3 cells treated with 1 was measured using the water-soluble tetrazolium 1 reagent. BXPC-3 cells induced by 1 were stained with diverse probes or antibodies, such as ethidium homodimer-1, Hoechst, anti-Ki67, and MitoTracker. Protein expression was measured using an immunoblotting assay, and mRNA expression was determined using real-time polymerase chain reaction. Apoptotic molecular features, such as lipid accumulation and protein degradation, were monitored directly using stimulated Raman scattering microspectroscopy. Through incubation time- and concentration-dependent studies of 1, we found that it significantly reduced the proliferation and increased the number of apoptotic BXPC-3 cells. Compound 1 induced mitochondrial dysfunction, phosphorylation of p38, and caspase 3 cleavage. These results indicate that 1 is a potential therapeutic agent for pancreatic cancer, providing valuable insights into the development of new anticancer drug candidates. © 2023 Elsevier Ltd | * |
dc.language | English | * |
dc.publisher | Elsevier Ltd | * |
dc.subject | Apoptosis | * |
dc.subject | Chalcone | * |
dc.subject | Lipid accumulation | * |
dc.subject | Pancreatic cancer | * |
dc.subject | Stimulated Raman scattering microscopy | * |
dc.title | A novel chalcone derivative exerts anticancer effects by promoting apoptotic cell death of human pancreatic cancer cells | * |
dc.type | Article | * |
dc.relation.volume | 93 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | Bioorganic and Medicinal Chemistry | * |
dc.identifier.doi | 10.1016/j.bmc.2023.117458 | * |
dc.identifier.wosid | WOS:001069200900001 | * |
dc.identifier.scopusid | 2-s2.0-85169306720 | * |
dc.author.google | Baek | * |
dc.author.google | Suji | * |
dc.author.google | Nah | * |
dc.author.google | Sanghee | * |
dc.author.google | Park | * |
dc.author.google | Joo Yeon | * |
dc.author.google | Lee | * |
dc.author.google | Sang Ju | * |
dc.author.google | Kang | * |
dc.author.google | Yong Gil | * |
dc.author.google | Kwon | * |
dc.author.google | Seung Hae | * |
dc.author.google | Oh | * |
dc.author.google | Seung Jun | * |
dc.author.google | Kang Pa | * |
dc.author.google | Moon | * |
dc.author.google | Byung Seok | * |
dc.contributor.scopusid | 문병석(22950995100;57206502981) | * |
dc.date.modifydate | 20240318142826 | * |