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Feasibility of18 F-Fluorocholine PET for Evaluating Skeletal Muscle Atrophy in a Starved Rat Model
- Title
- Feasibility of18 F-Fluorocholine PET for Evaluating Skeletal Muscle Atrophy in a Starved Rat Model
- Authors
- Park S.M.; Kim J.; Baek S.; Jeon J.-Y.; Lee S.J.; Kang S.Y.; Yoo M.Y.; Yoon H.-J.; Kwon S.H.; Lim K.; Oh S.J.; Kim B.S.; Lee K.P.; Moon B.S.
- Ewha Authors
- 김범산; 윤혜전; 이강파; 문병석; 강서영; 유민영
- SCOPUS Author ID
- 김범산; 윤혜전; 이강파; 문병석; 강서영; 유민영
- Issue Date
- 2022
- Journal Title
- Diagnostics
- ISSN
- 2075-4418
- Citation
- Diagnostics vol. 12, no. 5
- Keywords
- 18 F-Fluorocholine; atrogin-1; MuRF-1; positron emission tomography; skeletal muscle atrophy
- Publisher
- MDPI
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Imaging techniques for diagnosing muscle atrophy and sarcopenia remain insufficient, although various advanced diagnostic methods have been established. We explored the feasibility of18 F-fluorocholine (18 F-FCH) positron emission tomography/computed tomography (PET/CT) for evaluating skeletal muscle atrophy, as an imaging technique that tracks choline level changes in muscles. Cell uptake in L6 cells by18 F-FCH was performed in a complete medium containing serum (untreated group, UN) and a serum-free medium (starved group, ST). Small-animal-dedicated PET/CT imaging with18 F-FCH was examined in in-vivo models with rats that were starved for 2 days to cause muscle atrophy. After the hind limbs were dissected, starvation-induced in-vivo models were anatomically confirmed by reverse-transcription polymerase chain reaction to evaluate the expression levels of the atrophy markers muscle RING-finger protein-1 (MuRF-1) and atrogin-1.18 F-FCH uptake was lower in the starvation-induced cells than in the untreated group, and in-vivo PET uptake also revealed a similar tendency (the average standardized uptake value (SUVmean) = 0.26 ± 0.06 versus 0.37 ± 0.07, respectively). Furthermore, the expression levels of MuRF-1 and atrogin-1 mRNA were significantly increased in the starvation-induced muscle atrophy of rats compared to the untreated group.18 F-FCH PET/CT may be a promising tool for diagnosing skeletal muscle atrophy. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
- DOI
- 10.3390/diagnostics12051274
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
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