Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김범산 | * |
dc.contributor.author | 윤혜전 | * |
dc.contributor.author | 이강파 | * |
dc.contributor.author | 문병석 | * |
dc.contributor.author | 강서영 | * |
dc.contributor.author | 유민영 | * |
dc.date.accessioned | 2022-07-14T16:31:02Z | - |
dc.date.available | 2022-07-14T16:31:02Z | - |
dc.date.issued | 2022 | * |
dc.identifier.issn | 2075-4418 | * |
dc.identifier.other | OAK-31643 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/261532 | - |
dc.description.abstract | Imaging techniques for diagnosing muscle atrophy and sarcopenia remain insufficient, although various advanced diagnostic methods have been established. We explored the feasibility of18 F-fluorocholine (18 F-FCH) positron emission tomography/computed tomography (PET/CT) for evaluating skeletal muscle atrophy, as an imaging technique that tracks choline level changes in muscles. Cell uptake in L6 cells by18 F-FCH was performed in a complete medium containing serum (untreated group, UN) and a serum-free medium (starved group, ST). Small-animal-dedicated PET/CT imaging with18 F-FCH was examined in in-vivo models with rats that were starved for 2 days to cause muscle atrophy. After the hind limbs were dissected, starvation-induced in-vivo models were anatomically confirmed by reverse-transcription polymerase chain reaction to evaluate the expression levels of the atrophy markers muscle RING-finger protein-1 (MuRF-1) and atrogin-1.18 F-FCH uptake was lower in the starvation-induced cells than in the untreated group, and in-vivo PET uptake also revealed a similar tendency (the average standardized uptake value (SUVmean) = 0.26 ± 0.06 versus 0.37 ± 0.07, respectively). Furthermore, the expression levels of MuRF-1 and atrogin-1 mRNA were significantly increased in the starvation-induced muscle atrophy of rats compared to the untreated group.18 F-FCH PET/CT may be a promising tool for diagnosing skeletal muscle atrophy. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. | * |
dc.language | English | * |
dc.publisher | MDPI | * |
dc.subject | 18 F-Fluorocholine | * |
dc.subject | atrogin-1 | * |
dc.subject | MuRF-1 | * |
dc.subject | positron emission tomography | * |
dc.subject | skeletal muscle atrophy | * |
dc.title | Feasibility of18 F-Fluorocholine PET for Evaluating Skeletal Muscle Atrophy in a Starved Rat Model | * |
dc.type | Article | * |
dc.relation.issue | 5 | * |
dc.relation.volume | 12 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | Diagnostics | * |
dc.identifier.doi | 10.3390/diagnostics12051274 | * |
dc.identifier.wosid | WOS:000801692200001 | * |
dc.identifier.scopusid | 2-s2.0-85131098210 | * |
dc.author.google | Park S.M. | * |
dc.author.google | Kim J. | * |
dc.author.google | Baek S. | * |
dc.author.google | Jeon J.-Y. | * |
dc.author.google | Lee S.J. | * |
dc.author.google | Kang S.Y. | * |
dc.author.google | Yoo M.Y. | * |
dc.author.google | Yoon H.-J. | * |
dc.author.google | Kwon S.H. | * |
dc.author.google | Lim K. | * |
dc.author.google | Oh S.J. | * |
dc.author.google | Kim B.S. | * |
dc.author.google | Lee K.P. | * |
dc.author.google | Moon B.S. | * |
dc.contributor.scopusid | 김범산(35223582600) | * |
dc.contributor.scopusid | 윤혜전(47161772300) | * |
dc.contributor.scopusid | 이강파(57221725161) | * |
dc.contributor.scopusid | 문병석(22950995100;57206502981) | * |
dc.contributor.scopusid | 강서영(57203490211) | * |
dc.contributor.scopusid | 유민영(56924607400) | * |
dc.date.modifydate | 20240318142826 | * |