View : 164 Download: 0

Ethanol induces persistent potentiation of 5-HT3 receptor-stimulated GABA release at synapses on rat hippocampal CA1 neurons

Title
Ethanol induces persistent potentiation of 5-HT3 receptor-stimulated GABA release at synapses on rat hippocampal CA1 neurons
Authors
Jun, Sang BeomIkeda, Stephen R.Sung, Jee EunLovinger, David M.
Ewha Authors
성지은전상범
SCOPUS Author ID
성지은scopus; 전상범scopus
Issue Date
2021
Journal Title
NEUROPHARMACOLOGY
ISSN
0028-3908JCR Link

1873-7064JCR Link
Citation
NEUROPHARMACOLOGY vol. 184
Keywords
AlcoholGABAergic synapseSerotoninNeuron/boutonHippocampus
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Several studies have shown that ethanol (EtOH) can enhance the activity of GABAergic synapses via presynaptic mechanisms, including in hippocampal CA1 neurons. The serotonin type 3 receptor (5-HT3-R) has been implicated in the neural actions of ethanol (EtOH) and in modulation of GABA release from presynaptic terminals. In the present study, we investigated EtOH modulation of GABA release induced by 5-HT3-R activation using the mechanically isolated neuron/bouton preparation from the rat CA1 hippocampal subregion. EtOH application before and during exposure to the selective 5-HT3 receptor agonist, m-chlorophenylbiguanide (mCPBG) potentiated the mCPBG-induced increases in the peak frequency and charge transfer of spontaneous GABAergic inhibitory postsynaptic currents. Interestingly, the potentiation was maintained even after EtOH was removed from the preparation. A protein kinase A inhibitor reduced the magnitude of EtOH potentiation. Fluorescent Ca2+ imaging showed that Ca2+ transients in the presynaptic terminals increased during EtOH exposure. These findings indicate that EtOH produces long-lasting potentiation of 5-HT3-induced GABA release by modulating calcium levels, via a process involving cAMP-mediated signaling in presynaptic terminals.
Show the fulltext
DOI
10.1016/j.neuropharm.2020.108415
Appears in Collections:
사범대학 > 언어병리학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE