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Discrimination of Lycium chinense and L. barbarum Based on Metabolite Analysis and Hepatoprotective Activity
- Discrimination of Lycium chinense and L. barbarum Based on Metabolite Analysis and Hepatoprotective Activity
- Ryu M.-J.; Kim M.; Ji M.; Lee C.; Yang I.; Hong S.-B.; Chin J.; Seo E.K.; Paik M.-J.; Lim K.-M.; Nam S.-J.
- Ewha Authors
- 서은경; 임경민; 남상집; 김민정
- SCOPUS Author ID
- 서은경; 임경민; 남상집; 김민정
- Issue Date
- Journal Title
- Molecules (Basel, Switzerland)
- Molecules (Basel, Switzerland) vol. 25, no. 24
- gas chromatography-mass spectrometry; Lycium barbarum; Lycium chinense; star pattern recognition analysis
- NLM (Medline)
- SCIE; SCOPUS
- Document Type
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- Lycii Fructus is a traditional medicine used to prevent liver and kidney diseases, which commonly derives from Lycium chinense and Lycium barbarum. Here, the extracts and ethyl acetate-soluble fractions of L. chinense fruits exhibited better hepatoprotective effects than those of L. barbarum, which was likely due to differences in their composition. Therefore, GC-MS and HPLC analyses were conducted to characterize the metabolite differences between L. chinense and L. barbarum. Based on amino acid (AA) and phenolic acid (PA) profiling, 24 AAs and 9 PAs were identified in the two species. Moreover, each species exhibited unique and readily distinguishable AA and PA star graphic patterns. HPLC analysis elucidated composition differences between the ethyl acetate-soluble layers of the two compounds. Further, NMR analysis identified their chemical structures as 4-(2-formyl-5-(hydroxymethyl)-1H-pyrrol-1-yl)butanoic acid and p-coumaric acid. The higher content of 4-(2-formyl-5-(hydroxymethyl)-1H-pyrrol-1-yl)butanoic acid was detected in L. chinense, whereas the content of p-coumaric acid was higher in L. barbarum. Therefore, the differences in the relative contents of these two secondary metabolites in the ethyl acetate-soluble layer of Lycii Fructus could be a good marker to discriminate between L. chinense and L. barbarum.
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