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Discrimination of Lycium chinense and L. barbarum Based on Metabolite Analysis and Hepatoprotective Activity

Title
Discrimination of Lycium chinense and L. barbarum Based on Metabolite Analysis and Hepatoprotective Activity
Authors
Ryu M.-J.Kim M.Ji M.Lee C.Yang I.Hong S.-B.Chin J.Seo E.K.Paik M.-J.Lim K.-M.Nam S.-J.
Ewha Authors
서은경임경민남상집김민정
SCOPUS Author ID
서은경scopus; 임경민scopus; 남상집scopusscopus; 김민정scopus
Issue Date
2020
Journal Title
Molecules (Basel, Switzerland)
ISSN
1420-3049JCR Link
Citation
Molecules (Basel, Switzerland) vol. 25, no. 24
Keywords
gas chromatography-mass spectrometryLycium barbarumLycium chinensestar pattern recognition analysis
Publisher
NLM (Medline)
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Lycii Fructus is a traditional medicine used to prevent liver and kidney diseases, which commonly derives from Lycium chinense and Lycium barbarum. Here, the extracts and ethyl acetate-soluble fractions of L. chinense fruits exhibited better hepatoprotective effects than those of L. barbarum, which was likely due to differences in their composition. Therefore, GC-MS and HPLC analyses were conducted to characterize the metabolite differences between L. chinense and L. barbarum. Based on amino acid (AA) and phenolic acid (PA) profiling, 24 AAs and 9 PAs were identified in the two species. Moreover, each species exhibited unique and readily distinguishable AA and PA star graphic patterns. HPLC analysis elucidated composition differences between the ethyl acetate-soluble layers of the two compounds. Further, NMR analysis identified their chemical structures as 4-(2-formyl-5-(hydroxymethyl)-1H-pyrrol-1-yl)butanoic acid and p-coumaric acid. The higher content of 4-(2-formyl-5-(hydroxymethyl)-1H-pyrrol-1-yl)butanoic acid was detected in L. chinense, whereas the content of p-coumaric acid was higher in L. barbarum. Therefore, the differences in the relative contents of these two secondary metabolites in the ethyl acetate-soluble layer of Lycii Fructus could be a good marker to discriminate between L. chinense and L. barbarum.
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DOI
10.3390/molecules25245835
Appears in Collections:
약학대학 > 약학과 > Journal papers
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