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A Novel Method to Differentiate Tonsil-Derived Mesenchymal Stem Cells In Vitro into Estrogen-Secreting Cells

Title
A Novel Method to Differentiate Tonsil-Derived Mesenchymal Stem Cells In Vitro into Estrogen-Secreting Cells
Authors
Kim, Hee-YeonLee, YounghayYoon, Hee-SooKim, Yu-HeeCho, Kyong-AWoo, So-YounKim, Han SunPark, Bo-YoungJung, Sung-ChulJo, InhoPark, Woo-JaePark, Joo-WonRyu, Kyung-Ha
Ewha Authors
유경하우소연정성철조인호박주원김유희박보영조경아
SCOPUS Author ID
유경하scopus; 우소연scopus; 정성철scopus; 조인호scopus; 박주원scopus; 김유희scopus; 박보영scopus; 조경아scopus
Issue Date
2021
Journal Title
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
ISSN
1738-2696JCR Link

2212-5469JCR Link
Citation
TISSUE ENGINEERING AND REGENERATIVE MEDICINE vol. 18, no. 2, pp. 253 - 264
Keywords
EstrogenTonsilMesenchymal stem cellSecretionCytochrome P450 family 19 subfamily A member 1
Publisher
KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC
Indexed
SCIE; SCOPUS; KCI WOS scopus
Document Type
Article
Abstract
BACKGROUND: The advantages of tonsil-derived mesenchymal stem cells (TMSCs) over other mesenchymal stem cells (MSCs) include higher proliferation rates, various differentiation potentials, efficient immune-modulating capacity, and ease of obtainment. Specifically, TMSCs have been shown to differentiate into the endodermal lineage. Estrogen deficiency is a major cause of postmenopausal osteoporosis and is associated with higher incidences of ischemic heart disease and cerebrovascular attacks during the postmenopausal period. Therefore, stem cell-derived, estrogen-secreting cells might be used for estrogen deficiency. METHODS: Here, we developed a novel method that utilizes retinoic acid, insulin-like growth factor-1, basic fibroblast growth factor, and dexamethasone to evaluate the differentiating potential of TMSCs into estrogen-secreting cells. The efficacy of the novel differentiating method for generation of estrogen-secreting cells was also evaluated with bone marrow- and adipose tissue-derived MSCs. RESULTS: Incubating TMSCs in differentiating media induced the gene expression of cytochrome P450 19A1 (CYP19A1), which plays a key role in estrogen biosynthesis, and increased 17 beta-estradiol secretion upon testosterone addition. Furthermore, CYP11A1, CYP17A1, and 3 beta-hydroxysteroid dehydrogenase type-1 gene expression levels were significantly increased in TMSCs. In bone marrow-derived and adipose tissue-derived MSCs, this differentiation method also induced the gene expression of CYP19A1, but not CYP17A1, suggesting TMSCs are a superior source for estrogen secretion. CONCLUSION: These results imply that TMSCs can differentiate into functional estrogen-secreting cells, thus providing a novel, alternative cell therapy for estrogen deficiency.
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DOI
10.1007/s13770-020-00307-y
Appears in Collections:
의과대학 > 의학과 > Journal papers
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