Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 유경하 | * |
dc.contributor.author | 우소연 | * |
dc.contributor.author | 정성철 | * |
dc.contributor.author | 조인호 | * |
dc.contributor.author | 박주원 | * |
dc.contributor.author | 김유희 | * |
dc.contributor.author | 박보영 | * |
dc.contributor.author | 조경아 | * |
dc.date.accessioned | 2020-12-24T16:30:15Z | - |
dc.date.available | 2020-12-24T16:30:15Z | - |
dc.date.issued | 2021 | * |
dc.identifier.issn | 1738-2696 | * |
dc.identifier.issn | 2212-5469 | * |
dc.identifier.other | OAK-28529 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/255825 | - |
dc.description.abstract | BACKGROUND: The advantages of tonsil-derived mesenchymal stem cells (TMSCs) over other mesenchymal stem cells (MSCs) include higher proliferation rates, various differentiation potentials, efficient immune-modulating capacity, and ease of obtainment. Specifically, TMSCs have been shown to differentiate into the endodermal lineage. Estrogen deficiency is a major cause of postmenopausal osteoporosis and is associated with higher incidences of ischemic heart disease and cerebrovascular attacks during the postmenopausal period. Therefore, stem cell-derived, estrogen-secreting cells might be used for estrogen deficiency. METHODS: Here, we developed a novel method that utilizes retinoic acid, insulin-like growth factor-1, basic fibroblast growth factor, and dexamethasone to evaluate the differentiating potential of TMSCs into estrogen-secreting cells. The efficacy of the novel differentiating method for generation of estrogen-secreting cells was also evaluated with bone marrow- and adipose tissue-derived MSCs. RESULTS: Incubating TMSCs in differentiating media induced the gene expression of cytochrome P450 19A1 (CYP19A1), which plays a key role in estrogen biosynthesis, and increased 17 beta-estradiol secretion upon testosterone addition. Furthermore, CYP11A1, CYP17A1, and 3 beta-hydroxysteroid dehydrogenase type-1 gene expression levels were significantly increased in TMSCs. In bone marrow-derived and adipose tissue-derived MSCs, this differentiation method also induced the gene expression of CYP19A1, but not CYP17A1, suggesting TMSCs are a superior source for estrogen secretion. CONCLUSION: These results imply that TMSCs can differentiate into functional estrogen-secreting cells, thus providing a novel, alternative cell therapy for estrogen deficiency. | * |
dc.language | English | * |
dc.publisher | KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC | * |
dc.subject | Estrogen | * |
dc.subject | Tonsil | * |
dc.subject | Mesenchymal stem cell | * |
dc.subject | Secretion | * |
dc.subject | Cytochrome P450 family 19 subfamily A member 1 | * |
dc.title | A Novel Method to Differentiate Tonsil-Derived Mesenchymal Stem Cells In Vitro into Estrogen-Secreting Cells | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 18 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 253 | * |
dc.relation.lastpage | 264 | * |
dc.relation.journaltitle | TISSUE ENGINEERING AND REGENERATIVE MEDICINE | * |
dc.identifier.doi | 10.1007/s13770-020-00307-y | * |
dc.identifier.wosid | WOS:000584859300001 | * |
dc.identifier.scopusid | 2-s2.0-85094144327 | * |
dc.author.google | Kim, Hee-Yeon | * |
dc.author.google | Lee, Younghay | * |
dc.author.google | Yoon, Hee-Soo | * |
dc.author.google | Kim, Yu-Hee | * |
dc.author.google | Cho, Kyong-A | * |
dc.author.google | Woo, So-Youn | * |
dc.author.google | Kim, Han Sun | * |
dc.author.google | Park, Bo-Young | * |
dc.author.google | Jung, Sung-Chul | * |
dc.author.google | Jo, Inho | * |
dc.author.google | Park, Woo-Jae | * |
dc.author.google | Park, Joo-Won | * |
dc.author.google | Ryu, Kyung-Ha | * |
dc.contributor.scopusid | 유경하(14038236200) | * |
dc.contributor.scopusid | 우소연(7402853365) | * |
dc.contributor.scopusid | 정성철(57008539100) | * |
dc.contributor.scopusid | 조인호(26643129000;56663841900) | * |
dc.contributor.scopusid | 박주원(8656832200) | * |
dc.contributor.scopusid | 김유희(15764983100) | * |
dc.contributor.scopusid | 박보영(55516141700) | * |
dc.contributor.scopusid | 조경아(21734204400) | * |
dc.date.modifydate | 20240429113726 | * |