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Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation
- Title
- Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation
- Authors
- Moon, Hee Chul; Han, Sol; Borges, Joao; Pesqueira, Tamagno; Choi, Hyunwoo; Han, Sang Yeong; Cho, Hyeoncheol; Park, Ji Hun; Mano, Joao F.; Choi, Insung S.
- Ewha Authors
- 박지훈
- SCOPUS Author ID
- 박지훈
- Issue Date
- 2020
- Journal Title
- SOFT MATTER
- ISSN
- 1744-683X
1744-6848
- Citation
- SOFT MATTER vol. 16, no. 26, pp. 6063 - 6071
- Publisher
- ROYAL SOC CHEMISTRY
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- The build-up and degradation of cytocompatible nanofilms in a controlled fashion have great potential in biomedical and nanomedicinal fields, including single-cell nanoencapsulation (SCNE). Herein, we report the fabrication of biodegradable films of cationic starch (c-ST) and anionic alginate (ALG) by electrostatically driven layer-by-layer (LbL) assembly technology and its application to the SCNE. The [c-ST/ALG] multilayer nanofilms, assembled either on individual Saccharomyces cerevisiae or on the 2D flat gold surface, degrade on demand, in a cytocompatible fashion, via treatment with alpha-amylase. Their degradation profiles are investigated, while systematically changing the alpha-amylase concentration, by several surface characterization techniques, including quartz crystal microbalance with dissipation monitoring (QCM-D) and ellipsometry. DNA incorporation in the LbL nanofilms and its controlled release, upon exposure of the nanofilms to an aqueous alpha-amylase solution, are demonstrated. The highly cytocompatible nature of the film-forming and -degrading conditions is assessed in the c-ST/ALG-shell formation and degradation of S. cerevisiae. We envisage that the cytocompatible, enzymatic degradation of c-ST-based nanofilms paves the way for developing advanced biomedical devices with programmed dissolution in vivo.
- DOI
- 10.1039/d0sm00876a
- Appears in Collections:
- 사범대학 > 과학교육과 > Journal papers
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