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Comparison of Four Commercial Kits for Isolation of Urinary Cell-Free DNA and Sample Storage Conditions

Title
Comparison of Four Commercial Kits for Isolation of Urinary Cell-Free DNA and Sample Storage Conditions
Authors
Lee, Eun YoungLee, Eun-JuYoon, HanaLee, Dong HyeonKim, Kwang Hyun
Ewha Authors
이동현윤하나김광현
SCOPUS Author ID
이동현scopus; 윤하나scopus; 김광현scopusscopus
Issue Date
2020
Journal Title
DIAGNOSTICS
ISSN
2075-4418JCR Link
Citation
DIAGNOSTICS vol. 10, no. 4
Keywords
urinary cell DNAliquid biopsycommercial kit
Publisher
MDPI
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Urinary cell-free DNA (cfDNA) is an attractive body fluid for liquid biopsy. In this study, we compared the efficiencies of four commercial kits for urinary cell-free DNA (cfDNA) isolation and of various sample storage conditions. Urinary cfDNA was isolated from 10 healthy individuals using four commercial kits: QIAamp Circulating Nucleic Acid Kit (QC; Qiagen), MagMAX (TM) Cell-Free DNA Isolation Kit (MM; Applied Biosystems), Urine Cell-Free Circulating DNA Purification Midi Kit (NU; Norgen Biotek), and Quick-DNA (TM) Urine Kit (ZQ; Zymo Research). To assess the isolation efficiency, an Agilent 2100 Bioanalyzer with High Sensitivity DNA chips was used, and cfDNA yield was defined as the amount of cfDNA obtained from 1 mL of urine. MM and QC provided the highest cfDNA yield in the 50-300 bp range, and MM and NU gave the highest cfDNA yield in the 50-100 bp range. In particular, the NU kit was efficient for isolation of more fragmented cfDNA in the range of 50-100 bp with the lowest cellular genomic DNA contamination. ZQ had the best cost-efficiency for isolating the same amount of urinary cfDNA. Samples stored at -70 degrees C with the addition of 10 mM EDTA resulted in the highest cfDNA yield 3 months after sample collection.
DOI
10.3390/diagnostics10040234
Appears in Collections:
의과대학 > 의학과 > Journal papers
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