Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이동현 | * |
dc.contributor.author | 윤하나 | * |
dc.contributor.author | 김광현 | * |
dc.date.accessioned | 2020-06-01T16:30:24Z | - |
dc.date.available | 2020-06-01T16:30:24Z | - |
dc.date.issued | 2020 | * |
dc.identifier.issn | 2075-4418 | * |
dc.identifier.other | OAK-26917 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/253963 | - |
dc.description.abstract | Urinary cell-free DNA (cfDNA) is an attractive body fluid for liquid biopsy. In this study, we compared the efficiencies of four commercial kits for urinary cell-free DNA (cfDNA) isolation and of various sample storage conditions. Urinary cfDNA was isolated from 10 healthy individuals using four commercial kits: QIAamp Circulating Nucleic Acid Kit (QC; Qiagen), MagMAX (TM) Cell-Free DNA Isolation Kit (MM; Applied Biosystems), Urine Cell-Free Circulating DNA Purification Midi Kit (NU; Norgen Biotek), and Quick-DNA (TM) Urine Kit (ZQ; Zymo Research). To assess the isolation efficiency, an Agilent 2100 Bioanalyzer with High Sensitivity DNA chips was used, and cfDNA yield was defined as the amount of cfDNA obtained from 1 mL of urine. MM and QC provided the highest cfDNA yield in the 50-300 bp range, and MM and NU gave the highest cfDNA yield in the 50-100 bp range. In particular, the NU kit was efficient for isolation of more fragmented cfDNA in the range of 50-100 bp with the lowest cellular genomic DNA contamination. ZQ had the best cost-efficiency for isolating the same amount of urinary cfDNA. Samples stored at -70 degrees C with the addition of 10 mM EDTA resulted in the highest cfDNA yield 3 months after sample collection. | * |
dc.language | English | * |
dc.publisher | MDPI | * |
dc.subject | urinary cell DNA | * |
dc.subject | liquid biopsy | * |
dc.subject | commercial kit | * |
dc.title | Comparison of Four Commercial Kits for Isolation of Urinary Cell-Free DNA and Sample Storage Conditions | * |
dc.type | Article | * |
dc.relation.issue | 4 | * |
dc.relation.volume | 10 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | DIAGNOSTICS | * |
dc.identifier.doi | 10.3390/diagnostics10040234 | * |
dc.identifier.wosid | WOS:000534251000017 | * |
dc.identifier.scopusid | 2-s2.0-85083774993 | * |
dc.author.google | Lee, Eun Young | * |
dc.author.google | Lee, Eun-Ju | * |
dc.author.google | Yoon, Hana | * |
dc.author.google | Lee, Dong Hyeon | * |
dc.author.google | Kim, Kwang Hyun | * |
dc.contributor.scopusid | 이동현(24341048900;57202299352) | * |
dc.contributor.scopusid | 윤하나(8723844200) | * |
dc.contributor.scopusid | 김광현(57191527009) | * |
dc.date.modifydate | 20240222163024 | * |