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Engineering the intracellular metabolism of Escherichia coli to produce gamma-aminobutyric acid by co-localization of GABA shunt enzymes

Title
Engineering the intracellular metabolism of Escherichia coli to produce gamma-aminobutyric acid by co-localization of GABA shunt enzymes
Authors
Van Dung PhamSomasundaram, SivachandiranLee, Seung HwanPark, Si JaeHong, Soon Ho
Ewha Authors
박시재
SCOPUS Author ID
박시재scopus
Issue Date
2016
Journal Title
BIOTECHNOLOGY LETTERS
ISSN
0141-5492JCR Link

1573-6776JCR Link
Citation
BIOTECHNOLOGY LETTERS vol. 38, no. 2, pp. 321 - 327
Keywords
Gamma-aminobutyric acidGamma-aminobutyric acid shuntCo-localizationEscherichia coliGamma-Scaffold system
Publisher
SPRINGER
Indexed
SCIE; SCOPUS WOS
Document Type
Article
Abstract
To direct the carbon flux from Krebs cycle into the gamma-aminobutyric acid (GABA) shunt pathway for the production of GABA by protein scaffold introduction in Escherichia coli. Escherichia coli was engineered to produce GABA from glucose by the co-localization of enzymes succinate semialdehyde dehydrogenase (GadD), GABA aminotransferase (PuuE) and GABA transporter (GadC) by protein scaffold. 0.7 g GABA l(-1) was produced from 10 g glucose l(-1) while no GABA was produced in wild type E. coli. pH 6 and 30 A degrees C were optimum for GABA production, and GABA concentration increased to 1.12 g GABA l(-1) when 20 g glucose l(-1) was used. When competing metabolic networks were inactivated, GABA increased by 24 % (0.87 g GABA l(-1)). The novel GABA production system was constructed by co-localization of GABA shunt enzymes.
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DOI
10.1007/s10529-015-1982-2
Appears in Collections:
엘텍공과대학 > 화학신소재공학전공 > Journal papers
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