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dc.contributor.author박시재-
dc.date.accessioned2019-11-19T16:30:50Z-
dc.date.available2019-11-19T16:30:50Z-
dc.date.issued2016-
dc.identifier.issn0141-5492-
dc.identifier.issn1573-6776-
dc.identifier.otherOAK-25847-
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/252022-
dc.description.abstractTo direct the carbon flux from Krebs cycle into the gamma-aminobutyric acid (GABA) shunt pathway for the production of GABA by protein scaffold introduction in Escherichia coli. Escherichia coli was engineered to produce GABA from glucose by the co-localization of enzymes succinate semialdehyde dehydrogenase (GadD), GABA aminotransferase (PuuE) and GABA transporter (GadC) by protein scaffold. 0.7 g GABA l(-1) was produced from 10 g glucose l(-1) while no GABA was produced in wild type E. coli. pH 6 and 30 A degrees C were optimum for GABA production, and GABA concentration increased to 1.12 g GABA l(-1) when 20 g glucose l(-1) was used. When competing metabolic networks were inactivated, GABA increased by 24 % (0.87 g GABA l(-1)). The novel GABA production system was constructed by co-localization of GABA shunt enzymes.-
dc.languageEnglish-
dc.publisherSPRINGER-
dc.subjectGamma-aminobutyric acid-
dc.subjectGamma-aminobutyric acid shunt-
dc.subjectCo-localization-
dc.subjectEscherichia coli-
dc.subjectGamma-Scaffold system-
dc.titleEngineering the intracellular metabolism of Escherichia coli to produce gamma-aminobutyric acid by co-localization of GABA shunt enzymes-
dc.typeArticle-
dc.relation.issue2-
dc.relation.volume38-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage321-
dc.relation.lastpage327-
dc.relation.journaltitleBIOTECHNOLOGY LETTERS-
dc.identifier.doi10.1007/s10529-015-1982-2-
dc.identifier.wosidWOS:000370374300016-
dc.author.googleVan Dung Pham-
dc.author.googleSomasundaram, Sivachandiran-
dc.author.googleLee, Seung Hwan-
dc.author.googlePark, Si Jae-
dc.author.googleHong, Soon Ho-
dc.contributor.scopusid박시재(57191670770)-
dc.date.modifydate20210929141221-
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엘텍공과대학 > 화학신소재공학전공 > Journal papers
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