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Peroxiredoxin III Protects Tumor Suppressor PTEN from Oxidation by 15-Hydroperoxy-eicosatetraenoic Acid

Title
Peroxiredoxin III Protects Tumor Suppressor PTEN from Oxidation by 15-Hydroperoxy-eicosatetraenoic Acid
Authors
Zhang, YingPark, JiyoungHan, Seong-JeongLim, YongwoonPark, IhaKim, Jong-SukWoo, Hyun AeLee, Seung-Rock
Ewha Authors
우현애
SCOPUS Author ID
우현애scopus
Issue Date
2019
Journal Title
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
ISSN
1942-0900JCR Link

1942-0994JCR Link
Citation
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY vol. 2019
Publisher
HINDAWI LTD
Indexed
SCIE; SCOPUS WOS
Document Type
Article
Abstract
Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is a lipid and protein phosphatase that coordinates various cellular processes. Its activity is regulated by the reversible oxidation of an active-site cysteine residue by H2O2 and thioredoxin. However, the potential role of lipid peroxides in the redox regulation of PTEN remains obscure. To evaluate this, 15-hydroperoxy-eicosatetraenoic acid (15s-HpETE), a lipid peroxide, was employed to investigate its effect on PTEN using molecular and cellular-based assays. Exposure to 15s-HpETE resulted in the oxidation of recombinant PTEN. Reversible oxidation of PTEN was also observed in mouse embryonic fibroblast (MEF) cells treated with a 15s-HpETE and Lipofectamine mixture. The oxidative dimerization of thioredoxin was found simultaneously. In addition, the absence of peroxiredoxin III aggravated 15s-HpETE-induced PTEN oxidation in MEF cells. Our study provides novel insight into the mechanism linking lipid peroxidation to the etiology of tumorigenesis.
DOI
10.1155/2019/2828493
Appears in Collections:
약학대학 > 약학과 > Journal papers
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