View : 540 Download: 0
Improved production of clavulanic acid by reverse engineering and overexpression of the regulatory genes in an industrial Streptomyces clavuligerus strain
- Title
- Improved production of clavulanic acid by reverse engineering and overexpression of the regulatory genes in an industrial Streptomyces clavuligerus strain
- Authors
- Cho, Hang Soo; Jo, Jin Chul; Shin, Chang-Hun; Lee, Namil; Choi, Joon-Sun; Cho, Byung-Kwan; Roe, Jung-Hye; Kim, Chan-Wha; Kwon, Ho Jeong; Yoon, Yeo Joon
- Ewha Authors
- 윤여준
- SCOPUS Author ID
- 윤여준
- Issue Date
- 2019
- Journal Title
- JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
- ISSN
- 1367-5435
1476-5535
- Citation
- JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY vol. 46, no. 8, pp. 1205 - 1215
- Keywords
- Clavulanic acid; Streptomyces clavuligerus; Reverse engineering; Metabolic engineering
- Publisher
- SPRINGER HEIDELBERG
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Genomic analysis of the clavulanic acid (CA)-high-producing Streptomyces clavuligerus strains, OL13 and OR, developed through random mutagenesis revealed a frameshift mutation in the cas1 gene-encoding clavaminate synthase 1. Overexpression of the intact cas1 in S. clavuligerus OR enhanced the CA titer by approximately 25%, producing similar to 4.95 g/L of CA, over the OR strain in the flask culture. Moreover, overexpression of the pathway-specific positive regulatory genes, ccaR and claR, in the OR strain improved CA yield by approximately 43% (similar to 5.66 g/L) in the flask. However, co-expression of the intact cas1 with ccaR-claR did not further improve CA production. In the 7 L fermenter culture, maximum CA production by the OR strain expressing the wild-type cas1 and ccaR-claR reached approximately 5.52 g/L and 6.01 g/L, respectively, demonstrating that reverse engineering or simple rational metabolic engineering is an efficient method for further improvement of industrial strains.
- DOI
- 10.1007/s10295-019-02196-0
- Appears in Collections:
- 자연과학대학 > 화학·나노과학전공 > Journal papers
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML