Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤여준 | - |
dc.date.accessioned | 2019-10-02T01:59:59Z | - |
dc.date.available | 2019-10-02T01:59:59Z | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 1367-5435 | - |
dc.identifier.issn | 1476-5535 | - |
dc.identifier.other | OAK-25341 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/251475 | - |
dc.description.abstract | Genomic analysis of the clavulanic acid (CA)-high-producing Streptomyces clavuligerus strains, OL13 and OR, developed through random mutagenesis revealed a frameshift mutation in the cas1 gene-encoding clavaminate synthase 1. Overexpression of the intact cas1 in S. clavuligerus OR enhanced the CA titer by approximately 25%, producing similar to 4.95 g/L of CA, over the OR strain in the flask culture. Moreover, overexpression of the pathway-specific positive regulatory genes, ccaR and claR, in the OR strain improved CA yield by approximately 43% (similar to 5.66 g/L) in the flask. However, co-expression of the intact cas1 with ccaR-claR did not further improve CA production. In the 7 L fermenter culture, maximum CA production by the OR strain expressing the wild-type cas1 and ccaR-claR reached approximately 5.52 g/L and 6.01 g/L, respectively, demonstrating that reverse engineering or simple rational metabolic engineering is an efficient method for further improvement of industrial strains. | - |
dc.language | English | - |
dc.publisher | SPRINGER HEIDELBERG | - |
dc.subject | Clavulanic acid | - |
dc.subject | Streptomyces clavuligerus | - |
dc.subject | Reverse engineering | - |
dc.subject | Metabolic engineering | - |
dc.title | Improved production of clavulanic acid by reverse engineering and overexpression of the regulatory genes in an industrial Streptomyces clavuligerus strain | - |
dc.type | Article | - |
dc.relation.issue | 8 | - |
dc.relation.volume | 46 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 1205 | - |
dc.relation.lastpage | 1215 | - |
dc.relation.journaltitle | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.identifier.doi | 10.1007/s10295-019-02196-0 | - |
dc.identifier.wosid | WOS:000481796500014 | - |
dc.identifier.scopusid | 2-s2.0-85067066994 | - |
dc.author.google | Cho, Hang Soo | - |
dc.author.google | Jo, Jin Chul | - |
dc.author.google | Shin, Chang-Hun | - |
dc.author.google | Lee, Namil | - |
dc.author.google | Choi, Joon-Sun | - |
dc.author.google | Cho, Byung-Kwan | - |
dc.author.google | Roe, Jung-Hye | - |
dc.author.google | Kim, Chan-Wha | - |
dc.author.google | Kwon, Ho Jeong | - |
dc.author.google | Yoon, Yeo Joon | - |
dc.contributor.scopusid | 윤여준(7402126465) | - |
dc.date.modifydate | 20210708161345 | - |