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Volumetric Analysis of 3-D-Cultured Colonies in Wet Alginate Spots Using 384-Pillar Plate

Title
Volumetric Analysis of 3-D-Cultured Colonies in Wet Alginate Spots Using 384-Pillar Plate
Authors
Lee D.W.Choi Y.-J.Lee S.-Y.Kim M.-H.Doh I.Ryu G.H.Choi S.-M.
Ewha Authors
김명희
SCOPUS Author ID
김명희scopus
Issue Date
2018
Journal Title
SLAS Technology
ISSN
2472-6303JCR Link
Citation
SLAS Technology vol. 23, no. 3, pp. 226 - 230
Keywords
3-D cell cultureassay miniaturizationcell encapsulation with alginateclonogenic assayhigh-throughput screening
Publisher
SAGE Publications Inc.
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
The volumetric analysis of three-dimensional (3-D)-cultured colonies in alginate spots has been proposed to increase drug efficacy. In a previously developed pillar/well chip platform, colonies within spots are usually stained and dried for analysis of cell viability using two-dimensional (2-D) fluorescent images. Since the number of viable cells in colonies is directly related to colony volume, we proposed the 3-D analysis of colonies for high-accuracy cell viability calculation. The spots were immersed in buffer, and the 3-D volume of each colony was calculated from the 2-D stacking fluorescent images of the spot with different focal positions. In the experiments with human gastric carcinoma cells and anticancer drugs, we compared cell viability values calculated using the 2-D area and 3-D volume of colonies in the wet and dried alginate spots, respectively. The IC50 value calculated using the 3-D volume of the colonies (9.5 μM) was less than that calculated in the 2-D area analysis (121.5 μM). We observed that the colony showed a more sensitive drug response regarding volume calculated from the 3-D image reconstructed using several confocal images than regarding colony area calculated in the 2-D analysis. © 2017, © 2017 Society for Laboratory Automation and Screening.
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DOI
10.1177/2472630317737698
Appears in Collections:
엘텍공과대학 > 컴퓨터공학과 > Journal papers
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