Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김명희 | * |
dc.date.accessioned | 2018-12-14T16:31:00Z | - |
dc.date.available | 2018-12-14T16:31:00Z | - |
dc.date.issued | 2018 | * |
dc.identifier.issn | 2472-6303 | * |
dc.identifier.other | OAK-22550 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/247779 | - |
dc.description.abstract | The volumetric analysis of three-dimensional (3-D)-cultured colonies in alginate spots has been proposed to increase drug efficacy. In a previously developed pillar/well chip platform, colonies within spots are usually stained and dried for analysis of cell viability using two-dimensional (2-D) fluorescent images. Since the number of viable cells in colonies is directly related to colony volume, we proposed the 3-D analysis of colonies for high-accuracy cell viability calculation. The spots were immersed in buffer, and the 3-D volume of each colony was calculated from the 2-D stacking fluorescent images of the spot with different focal positions. In the experiments with human gastric carcinoma cells and anticancer drugs, we compared cell viability values calculated using the 2-D area and 3-D volume of colonies in the wet and dried alginate spots, respectively. The IC50 value calculated using the 3-D volume of the colonies (9.5 μM) was less than that calculated in the 2-D area analysis (121.5 μM). We observed that the colony showed a more sensitive drug response regarding volume calculated from the 3-D image reconstructed using several confocal images than regarding colony area calculated in the 2-D analysis. © 2017, © 2017 Society for Laboratory Automation and Screening. | * |
dc.language | English | * |
dc.publisher | SAGE Publications Inc. | * |
dc.subject | 3-D cell culture | * |
dc.subject | assay miniaturization | * |
dc.subject | cell encapsulation with alginate | * |
dc.subject | clonogenic assay | * |
dc.subject | high-throughput screening | * |
dc.title | Volumetric Analysis of 3-D-Cultured Colonies in Wet Alginate Spots Using 384-Pillar Plate | * |
dc.type | Article | * |
dc.relation.issue | 3 | * |
dc.relation.volume | 23 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 226 | * |
dc.relation.lastpage | 230 | * |
dc.relation.journaltitle | SLAS Technology | * |
dc.identifier.doi | 10.1177/2472630317737698 | * |
dc.identifier.wosid | WOS:000432719200004 | * |
dc.identifier.scopusid | 2-s2.0-85042231644 | * |
dc.author.google | Lee D.W. | * |
dc.author.google | Choi Y.-J. | * |
dc.author.google | Lee S.-Y. | * |
dc.author.google | Kim M.-H. | * |
dc.author.google | Doh I. | * |
dc.author.google | Ryu G.H. | * |
dc.author.google | Choi S.-M. | * |
dc.contributor.scopusid | 김명희(34770838100) | * |
dc.date.modifydate | 20240322133114 | * |