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Keratinocytes negatively regulate the N-cadherin levels of melanoma cells via contact-mediated calcium regulation
- Title
- Keratinocytes negatively regulate the N-cadherin levels of melanoma cells via contact-mediated calcium regulation
- Authors
- Chung, Heesung; Jung, Hyejung; Jho, Eek-hoon; Multhaupt, Hinke A. B.; Couchman, John R.; Oh, Eok-Soo
- Ewha Authors
- 오억수; 정희성
- SCOPUS Author ID
- 오억수; 정희성
- Issue Date
- 2018
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- ISSN
- 0006-291X
1090-2104
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS vol. 503, no. 2, pp. 615 - 620
- Keywords
- Melanoma; Melanocyte; Keratinocyte; N-cadherin; Cell-cell interaction
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- In human skin, melanocytes and their neighboring keratinocytes have a close functional interrelationship. Keratinocytes, which represent the prevalent cell type of human skin, regulate melanocytes through various mechanisms. Here, we use a keratinocyte and melanoma co-culture system to show for the first time that keratinocytes regulate the cell surface expression of N-cadherin through cell-cell contact. Compared to mono-cultured human melanoma A375 cells, which expressed high levels of N-cadherin, those co-cultured with the HaCaT human keratinocyte cell line showed reduced levels of N-cadherin. This reduction was most evident in areas of A375 cells that underwent cell-cell contact with the HaCaT cells, whereas HaCaT cell-derived extracellular matrix and conditioned medium both failed to reduce Ncadherin levels. The intracellular level of calcium in co-cultured A375 cells was lower than that in mono cultured A375 cells, and treatment with a cell-permeant calcium chelator (BAPTA) reduced the N-cadherin level of mono-cultured A375 cells. Furthermore, co-culture with HaCaT cells reduced the expression levels of transient receptor potential cation channel (TRPC) 1, 3 and -6 in A375 cells, and siRNAmediated multi-depletion of TRPC1,-3 and -6 reduced the N-cadherin level in these cells. Taken together, these data suggest that keratinocytes negatively regulate the N-cadherin levels of melanoma cells via cell-to-cell contact-mediated calcium regulation. (C) 2018 Elsevier Inc. All rights reserved.
- DOI
- 10.1016/j.bbrc.2018.06.050
- Appears in Collections:
- 자연과학대학 > 생명과학전공 > Journal papers
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