Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 오억수 | - |
dc.contributor.author | 정희성 | - |
dc.date.accessioned | 2018-12-14T16:30:26Z | - |
dc.date.available | 2018-12-14T16:30:26Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.other | OAK-22875 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/247584 | - |
dc.description.abstract | In human skin, melanocytes and their neighboring keratinocytes have a close functional interrelationship. Keratinocytes, which represent the prevalent cell type of human skin, regulate melanocytes through various mechanisms. Here, we use a keratinocyte and melanoma co-culture system to show for the first time that keratinocytes regulate the cell surface expression of N-cadherin through cell-cell contact. Compared to mono-cultured human melanoma A375 cells, which expressed high levels of N-cadherin, those co-cultured with the HaCaT human keratinocyte cell line showed reduced levels of N-cadherin. This reduction was most evident in areas of A375 cells that underwent cell-cell contact with the HaCaT cells, whereas HaCaT cell-derived extracellular matrix and conditioned medium both failed to reduce Ncadherin levels. The intracellular level of calcium in co-cultured A375 cells was lower than that in mono cultured A375 cells, and treatment with a cell-permeant calcium chelator (BAPTA) reduced the N-cadherin level of mono-cultured A375 cells. Furthermore, co-culture with HaCaT cells reduced the expression levels of transient receptor potential cation channel (TRPC) 1, 3 and -6 in A375 cells, and siRNAmediated multi-depletion of TRPC1,-3 and -6 reduced the N-cadherin level in these cells. Taken together, these data suggest that keratinocytes negatively regulate the N-cadherin levels of melanoma cells via cell-to-cell contact-mediated calcium regulation. (C) 2018 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | Melanoma | - |
dc.subject | Melanocyte | - |
dc.subject | Keratinocyte | - |
dc.subject | N-cadherin | - |
dc.subject | Cell-cell interaction | - |
dc.title | Keratinocytes negatively regulate the N-cadherin levels of melanoma cells via contact-mediated calcium regulation | - |
dc.type | Article | - |
dc.relation.issue | 2 | - |
dc.relation.volume | 503 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 615 | - |
dc.relation.lastpage | 620 | - |
dc.relation.journaltitle | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.identifier.doi | 10.1016/j.bbrc.2018.06.050 | - |
dc.identifier.wosid | WOS:000442711200032 | - |
dc.identifier.scopusid | 2-s2.0-85048439102 | - |
dc.author.google | Chung, Heesung | - |
dc.author.google | Jung, Hyejung | - |
dc.author.google | Jho, Eek-hoon | - |
dc.author.google | Multhaupt, Hinke A. B. | - |
dc.author.google | Couchman, John R. | - |
dc.author.google | Oh, Eok-Soo | - |
dc.contributor.scopusid | 오억수(7101967153) | - |
dc.contributor.scopusid | 정희성(35106799300) | - |
dc.date.modifydate | 20230210132906 | - |