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Patterns of rpoC mutations in drug-resistant mycobacterium tuberculosis isolated from patients in South Korea
- Title
- Patterns of rpoC mutations in drug-resistant mycobacterium tuberculosis isolated from patients in South Korea
- Authors
- Yun Y.J.; Lee J.S.; Yoo J.C.; Cho E.; Park D.; Kook Y.-H.; Lee K.H.
- Ewha Authors
- 윤여준
- SCOPUS Author ID
- 윤여준
- Issue Date
- 2018
- Journal Title
- Tuberculosis and Respiratory Diseases
- ISSN
- 1738-3536
- Citation
- Tuberculosis and Respiratory Diseases vol. 81, no. 3, pp. 222 - 227
- Keywords
- Beta' subunit of RNA polymerase; Drug resistance; Multiple; Mutation; Mycobacterium tuberculosis
- Publisher
- Korean National Tuberculosis Association
- Indexed
- SCOPUS; KCI
- Document Type
- Article
- Abstract
- Background: Rifampicin (RFP) is one of the principal first-line drugs used in combination chemotherapies against Mycobacterium tuberculosis, and its use has greatly shortened the duration of chemotherapy for the successful treatment of drug-susceptible tuberculosis. Compensatory mutations have been identified in rpoC that restore the fitness of RFP-resistant M. tuberculosis strains with mutations in rpoB. To investigate rpoC mutation patterns, we analyzed 93 clinical M. tuberculosis isolates from patients in South Korea. Methods: Drug-resistant mycobacterial isolates were cultured to determine their susceptibility to anti-tubercular agents. Mutations in rpoC were identified by sequencing and compared with the relevant wild-type DNA sequence. Results: In total, 93 M. tuberculosis clinical isolates were successfully cultured and tested for drug susceptibilities. They included 75 drug-resistant tuberculosis species, of which 66 were RFP-resistant strains. rpoC mutations were found in 24 of the 66 RFP-resistant isolates (36.4%). Fifteen different types of mutations, including single mutations (22/24, 91.7%) and multiple mutations (2/24, 8.3%), were identified, and 12 of these mutations are reported for the first time in this study. The most frequent mutation involved a substitution at codon 452 (nt 1356) resulting in amino acid change F452L. Conclusion: Fifteen different types of mutations were identified and were predominantly single-nucleotide substitutions (91.7%). Mutations were found only in dual isoniazid- and RFP-resistant isolates of M. tuberculosis. No mutations were identified in any of the drug-susceptible strains. Copyright © 2018 The Korean Academy of Tuberculosis and Respiratory Diseases.
- DOI
- 10.4046/trd.2017.0042
- Appears in Collections:
- 연구기관 > 의과학연구소 > Journal papers
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