Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 윤여준 | - |
dc.date.accessioned | 2018-11-16T16:30:16Z | - |
dc.date.available | 2018-11-16T16:30:16Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 1738-3536 | - |
dc.identifier.other | OAK-23441 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/246598 | - |
dc.description.abstract | Background: Rifampicin (RFP) is one of the principal first-line drugs used in combination chemotherapies against Mycobacterium tuberculosis, and its use has greatly shortened the duration of chemotherapy for the successful treatment of drug-susceptible tuberculosis. Compensatory mutations have been identified in rpoC that restore the fitness of RFP-resistant M. tuberculosis strains with mutations in rpoB. To investigate rpoC mutation patterns, we analyzed 93 clinical M. tuberculosis isolates from patients in South Korea. Methods: Drug-resistant mycobacterial isolates were cultured to determine their susceptibility to anti-tubercular agents. Mutations in rpoC were identified by sequencing and compared with the relevant wild-type DNA sequence. Results: In total, 93 M. tuberculosis clinical isolates were successfully cultured and tested for drug susceptibilities. They included 75 drug-resistant tuberculosis species, of which 66 were RFP-resistant strains. rpoC mutations were found in 24 of the 66 RFP-resistant isolates (36.4%). Fifteen different types of mutations, including single mutations (22/24, 91.7%) and multiple mutations (2/24, 8.3%), were identified, and 12 of these mutations are reported for the first time in this study. The most frequent mutation involved a substitution at codon 452 (nt 1356) resulting in amino acid change F452L. Conclusion: Fifteen different types of mutations were identified and were predominantly single-nucleotide substitutions (91.7%). Mutations were found only in dual isoniazid- and RFP-resistant isolates of M. tuberculosis. No mutations were identified in any of the drug-susceptible strains. Copyright © 2018 The Korean Academy of Tuberculosis and Respiratory Diseases. | - |
dc.description.sponsorship | Jeju National University Hospital | - |
dc.language | English | - |
dc.publisher | Korean National Tuberculosis Association | - |
dc.subject | Beta&apos | - |
dc.subject | subunit of RNA polymerase | - |
dc.subject | Drug resistance | - |
dc.subject | Multiple | - |
dc.subject | Mutation | - |
dc.subject | Mycobacterium tuberculosis | - |
dc.title | Patterns of rpoC mutations in drug-resistant mycobacterium tuberculosis isolated from patients in South Korea | - |
dc.type | Article | - |
dc.relation.issue | 3 | - |
dc.relation.volume | 81 | - |
dc.relation.index | SCOPUS | - |
dc.relation.index | KCI | - |
dc.relation.startpage | 222 | - |
dc.relation.lastpage | 227 | - |
dc.relation.journaltitle | Tuberculosis and Respiratory Diseases | - |
dc.identifier.doi | 10.4046/trd.2017.0042 | - |
dc.identifier.scopusid | 2-s2.0-85050496296 | - |
dc.author.google | Yun Y.J. | - |
dc.author.google | Lee J.S. | - |
dc.author.google | Yoo J.C. | - |
dc.author.google | Cho E. | - |
dc.author.google | Park D. | - |
dc.author.google | Kook Y.-H. | - |
dc.author.google | Lee K.H. | - |
dc.contributor.scopusid | 윤여준(36462140900) | - |
dc.date.modifydate | 20181116160146 | - |