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Analysis of bacterial community structure in leachate-contaminated groundwater using denaturing gradient gel electrophoresis
- Analysis of bacterial community structure in leachate-contaminated groundwater using denaturing gradient gel electrophoresis
- Kim J.; Kim J.-Y.; Koo S.-Y.; Ko K.-S.; Lee S.D.; Cho K.-S.; Koh D.-C.
- Ewha Authors
- 조경숙; 이상돈
- SCOPUS Author ID
- 조경숙; 이상돈
- Issue Date
- Journal Title
- Korean Journal of Microbiology and Biotechnology
- Korean Journal of Microbiology and Biotechnology vol. 34, no. 2, pp. 166 - 173
- SCOPUS; KCI
- Document Type
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- This research has been performed to clarify the relationship between hydrogeochemistry and bacterial community structure in ground-water contaminated with landfill leachate. We collected and analyzed samples from 5 sites such as leachate (KSG1-12), treated leachate (KSG1-16), two contaminated groundwaters (KSG1-07 and KSG1-08) and non-contaminated groundwater (KSG1-13). pH was 8.83, 8.04, 6.87, 6.87 and 6.53 in order; redox potential (Eh) 108, 202, 47, 200 and 154 mV; electric conductivity (EC) 3710, 894, 1223, 559 and 169.9 μS/cm; suspended solids (SS) 86.45, 13.74, 4.18, 0.24 and 11.91 mg/L. In KSG01-12, the ion concentrations were higher especially in Cl- and HCO 3 - than other sites. The concentrations of Fe, Mn and SO4 2- were higher in KSG1-07 than in KSG1-08, and vise versa in NO3 2-. In the comparison of DGGE fingerprint patterns, the similarity was highest between KSG1-13 and KSG1-16 (57.2%), probably due to common properties like low or none contaminant concentrations. Otherwise KSG1-08 showed lowest similarities with KSG1-13 (25.8%) and KSG1-12 (27.6%), maybe because of the degree of contamination. The most dominant bacterial species in each site were involved in α-Proteobacteria (55.6%) in KSG1-12, γ-Proteobacteria (50.0%) in KSG1-16, β-Proteobacteria (66.7%) in KSG1-07, α-Proteobacteria (54.5%) in KSG1-08 and β-Proteobacteria (36.4%) in KSG1-13. These results indicate that the microbial community structure might be changed according to the flow of leachate in grounderwater, implying changes in concentrations of pollutants, available electron acceptors and/or other environmental conditions.
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