Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 조경숙 | * |
dc.contributor.author | 김태관 | * |
dc.date.accessioned | 2016-08-29T12:08:00Z | - |
dc.date.available | 2016-08-29T12:08:00Z | - |
dc.date.issued | 2014 | * |
dc.identifier.issn | 2215-017X | * |
dc.identifier.other | OAK-14791 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/230522 | - |
dc.description.abstract | Two different quantitative PCR platforms, droplet digital PCR (dd-PCR) and quantitative real-time PCR (qPCR), were compared in a mcrA-based methanogen community assay that quantifies ten methanogen sub-groups. Both technologies exhibited similar PCR efficiencies over at least four orders of magnitude and the same lower limits of detection (8 copiesmL-DNA extract-1). The mcrA-based methanogen communities in three full-scale anaerobic digesters were examined using the two technologies. dd-PCR detected seven groups from the digesters, while qPCR did five groups, indicating that dd-PCR is more sensitive for DNA quantification. Linear regression showed quantitative agreements between both of the technologies (R2 = 0.59-0.98) in the five groups that were concurrently detected. Principal component analysis from the two datasets consistently indicated a substantial difference in the community composition among the digesters and revealed similar levels of differentiation among the communities. The combined results suggest that dd-PCR is more promising for examining methanogenic archaeal communities in biotechnological processes. © 2014 The Authors. Published by Elsevier B.V. | * |
dc.language | English | * |
dc.publisher | Elsevier | * |
dc.subject | Anaerobic digester | * |
dc.subject | Droplet-digital PCR | * |
dc.subject | mcrA | * |
dc.subject | Methanogen community | * |
dc.subject | Quantitative real-time PCR | * |
dc.title | Comparison of droplet digital PCR and quantitative real-time PCR in mcrA-based methanogen community analysis | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 4 | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1 | * |
dc.relation.lastpage | 4 | * |
dc.relation.journaltitle | Biotechnology Reports | * |
dc.identifier.doi | 10.1016/j.btre.2014.06.010 | * |
dc.identifier.scopusid | 2-s2.0-84926385157 | * |
dc.author.google | Kim T.G. | * |
dc.author.google | Jeong S.-Y. | * |
dc.author.google | Cho K.-S. | * |
dc.contributor.scopusid | 조경숙(7403957095) | * |
dc.contributor.scopusid | 김태관(57211805072;55151157100) | * |
dc.date.modifydate | 20240322131338 | * |